Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses
Autor: | Jikun Liu, Marie L. Landry, Indira Hewlett, Viswanath Ragupathy, Xue Wang, Zhiping Ye, Sai V. Vemula, Haja Sittana El Mubarak, Jiangqin Zhao |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Microbiology (medical)
Genotype Epidemiology Orthomyxoviridae Hemagglutinin (influenza) lcsh:Medicine Neuraminidase Hemagglutinin Glycoproteins Influenza Virus Biology DNA sequencing influenza virus lcsh:Infectious and parasitic diseases subtype Pandemic Multiplex polymerase chain reaction Influenza Human Humans Nanotechnology Multiplex lcsh:RC109-216 viruses hemagglutinin influenza A genes Phylogeny Oligonucleotide Array Sequence Analysis Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses Research lcsh:R fungi virus diseases food and beverages High-Throughput Nucleotide Sequencing DNA Amplicon biology.organism_classification Virology Infectious Diseases biology.protein nanomicroarray RNA next-generation sequencing influenza Multiplex Polymerase Chain Reaction |
Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 21, Iss 3, Pp 400-408 (2015) |
ISSN: | 1080-6059 1080-6040 |
Popis: | This novel platform can detect and differentiate different influenza subtypes from a single sample. Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics. |
Databáze: | OpenAIRE |
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