Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

Autor: Jikun Liu, Marie L. Landry, Indira Hewlett, Viswanath Ragupathy, Xue Wang, Zhiping Ye, Sai V. Vemula, Haja Sittana El Mubarak, Jiangqin Zhao
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Microbiology (medical)
Genotype
Epidemiology
Orthomyxoviridae
Hemagglutinin (influenza)
lcsh:Medicine
Neuraminidase
Hemagglutinin Glycoproteins
Influenza Virus

Biology
DNA sequencing
influenza virus
lcsh:Infectious and parasitic diseases
subtype
Pandemic
Multiplex polymerase chain reaction
Influenza
Human

Humans
Nanotechnology
Multiplex
lcsh:RC109-216
viruses
hemagglutinin
influenza A
genes
Phylogeny
Oligonucleotide Array Sequence Analysis
Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses
Research
lcsh:R
fungi
virus diseases
food and beverages
High-Throughput Nucleotide Sequencing
DNA
Amplicon
biology.organism_classification
Virology
Infectious Diseases
biology.protein
nanomicroarray
RNA
next-generation sequencing
influenza
Multiplex Polymerase Chain Reaction
Zdroj: Emerging Infectious Diseases
Emerging Infectious Diseases, Vol 21, Iss 3, Pp 400-408 (2015)
ISSN: 1080-6059
1080-6040
Popis: This novel platform can detect and differentiate different influenza subtypes from a single sample.
Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.
Databáze: OpenAIRE
Nepřihlášeným uživatelům se plný text nezobrazuje
načítá se...