Potential non-T cells source of interleukin-4 in food allergy
| Autor: | Madhan Masilamani, Hugh A. Sampson, Jean-Christoph Roger J-P Caubet, Lloyd Mayer, Neisha A Rivers |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Cell type Adolescent Antigens CD63/analysis Immunology Population Antigen presentation Cell Basophils/immunology Peripheral blood mononuclear cell Article Food allergy Immunology and Allergy Medicine Humans IL-2 receptor education Child Interleukin 4 education.field_of_study Antigen Presentation ddc:618 business.industry Tetraspanin 30 Interleukin-4/biosynthesis Interleukin-2 Receptor alpha Subunit Infant medicine.disease 3. Good health Basophils medicine.anatomical_structure Interleukin-2 Receptor alpha Subunit/analysis Child Preschool Pediatrics Perinatology and Child Health Female Interleukin-4 Food Hypersensitivity/immunology business Food Hypersensitivity |
| Zdroj: | Pediatric Allergy and Immunology, Vol. 25, No 3 (2014) pp. 243-9 |
| ISSN: | 1399-3038 0905-6157 |
| Popis: | Recently, a study from the Consortium of Food Allergy Research (CoFAR) showed that allergen-induced IL-4 expression in CD25(+) mononuclear cells was increased in allergic patients. However, they did not find the expected increase in GATA-3 expression, suggesting that allergen-induced IL-4 might not be of T-cell origin. We sought to determine whether other cell types were responsible for the increased IL-4 expression in the CD25(+) cell population.Comparing six allergic patients and six healthy controls, we analyzed the CD25(+) isolated population from PBMC for the presence of potential IL-4-expressing non-T cells. We also compared spontaneous expression levels of surface markers (CD203c, CD63, CD25, and HLA-DR) on basophils from whole blood of 42 peanut-allergic patients and from 12 non-atopic controls. Expression of these markers was also evaluated following basophil activation in eight peanut-allergic patients selected from the previous cohort.In addition to CD4(+) T cells, a substantial proportion of non-T cells were found in the CD25(+) -isolated cell population: basophils, NK, and NK-T cells with a mean percentage ± s.e.m. of 5.24 ± 0.63%, 6.65 ± 1.01%, and 6.01 ± 1.04%, respectively. The majority of these cells exhibited positive intracytoplasmic staining for IL-4. Expression of CD63 and CD25 was significantly higher in allergic patients compared with controls (p0.05). Interestingly, we found a significantly higher proportion of activated basophils expressing HLA-DR, compared with non-activated basophils (p0.05).Our results support the suggested key role of non-T cells secreting IL-4 in food allergy, particularly basophils, which may also play a central role in antigen presentation. |
| Databáze: | OpenAIRE |
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