Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples
| Autor: | M. Lean, Claire E. Wylie, A. Barrelet, N. A. Jocelyn, A. K. Foote |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Neutrophils 040301 veterinary sciences Cross-sectional study medicine.disease_cause Gastroenterology 0403 veterinary science 03 medical and health sciences Cytology Internal medicine Animals Medicine Horses Retrospective Studies Asthma Tracheal Diseases Bacteria business.industry Respiratory disease Age Factors Horse Pathogenic bacteria 04 agricultural and veterinary sciences General Medicine respiratory system medicine.disease Neutrophilia Trachea Cross-Sectional Studies Logistic Models 030104 developmental biology Multivariate Analysis Horse Diseases Seasons Sample collection medicine.symptom business |
| Zdroj: | Equine Veterinary Journal. 50:752-758 |
| ISSN: | 0425-1644 |
| DOI: | 10.1111/evj.12837 |
| Popis: | BACKGROUND Tracheal wash sample neutrophilia is common in lower airway inflammation of various causes; however, relevance of cytomorphological features to culture of bacterial pathogens has not been established. OBJECTIVES To investigate whether the presence of nondegenerate or degenerate neutrophils in tracheal washes is associated with culture of bacteria and, if so, whether this is influenced by age or temporal factors. STUDY DESIGN Cross-sectional study. METHODS Tracheal wash samples submitted to Rossdales LLP from 1/1/2013 to 31/7/2015 were evaluated using set criteria. Neutrophilia and degenerate neutrophilia (graded ≥2/4 on Rossdales cytological scale [0-4]) were analysed in relation to bacterial isolates considered potentially pathogenic in respiratory disease. Statistical analyses included multivariable logistic regression to identify associations between two separate outcomes: 1) the presence of neutrophilia compared with no neutrophilia and 2) the presence of degenerate neutrophilia compared with nondegenerate neutrophilia and four independent variables. RESULTS Sufficient data for inclusion in the multivariable model for nondegenerate neutrophilia were available from 1100 horses. Culture of potentially pathogenic bacteria was associated with increased odds of degenerate neutrophilia compared with samples with negative culture (OR 4.5, 95% CI 3.1, 6.4, P-value |
| Databáze: | OpenAIRE |
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