Interleukin-1β activates focal adhesion kinase and Src to induce matrix metalloproteinase-9 production and invasion of MCF-7 breast cancer cells
| Autor: | Takeshi Senga, Naing Naing Mon, Satoko Ito |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Oncogene Kinase Cell Articles Biology Cell cycle Cell biology Focal adhesion 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Oncology Cell culture Tumor progression Cancer research medicine biological phenomena cell phenomena and immunity Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Oncology Letters. 13:955-960 |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2016.5521 |
| Popis: | Interleukin-1β (IL-1b) is a pleiotropic cytokine that is important in tumor progression and invasion. Matrix metalloproteinase-9 (MMP-9), which is a secreted matrix-degrading enzyme, is one of the key regulators of tumor invasion and metastasis. The current report indicated that IL-1b promotes MMP-9 production and cell invasion in non-metastatic MCF-7 breast cancer cells. IL-1b activated focal adhesion kinase (FAK) and proto-oncogene tyrosine-protein kinase Src (Src). Moreover, inhibiting the Src/FAK pathway reduced the IL-1b-induced production of MMP-9 and cell invasion. To investigate the functional role of FAK in MMP-9 production cell lines expressing mutant FAK in FAK knock-out mouse fibroblasts were generated. In wild-type FAK-expressing cells, MMP-9 production was induced by IL-1b stimulation. By contrast, IL-1b-induced MMP-9 production was abrogated in FAK knock-out, FAK Y397F, FAK Y925F, and kinase dead mutant-expressing cells. Therefore the results of the current study indicate that FAK and Src kinases are activated by IL-1b and play a critical role in MMP-9 production and tumor cell invasion. |
| Databáze: | OpenAIRE |
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