Epithelial colonies in vitro elongate through collective effects
| Autor: | S.S. Soumya, Frank Jülicher, Guillaume Salbreux, S Anvitha, Daniel Riveline, Jordi Comelles, Linjie Lu, Mandar M. Inamdar, Emilie Le Maout |
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| Přispěvatelé: | Riveline, Daniel, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Science et d'ingénierie supramoléculaires (ISIS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Indian Institute of Technology Bombay (IIT Bombay), The Francis Crick Institute [London], Max Planck Institute for the Physics of Complex Systems (MPI-PKS), Max-Planck-Gesellschaft, Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell division [SDV]Life Sciences [q-bio] Cell 01 natural sciences symmetry breaking Epithelium Madin Darby Canine Kidney Cells [PHYS] Physics [physics] Cell elongation physics of living systems Morphogenesis Biology (General) Physics [PHYS]Physics [physics] 0303 health sciences Chemistry General Neuroscience 030302 biochemistry & molecular biology General Medicine Biomechanical Phenomena [SDV] Life Sciences [q-bio] medicine.anatomical_structure Medicine Elongation Cell Division Research Article Morphogen vertex model QH301-705.5 [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Science General Biochemistry Genetics and Molecular Biology Adherens junction 03 medical and health sciences Dogs tissue elongation 0103 physical sciences medicine Animals Humans Symmetry breaking 010306 general physics Process (anatomy) 030304 developmental biology Boundary cell General Immunology and Microbiology Epithelial Cells Global symmetry In vitro 030104 developmental biology Biophysics Other Caco-2 Cells |
| Zdroj: | eLife eLife, 2021, 10, ⟨10.7554/eLife.57730⟩ eLife, Vol 10 (2021) eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.57730⟩ eLife, eLife Sciences Publication, 2020, ⟨10.7554/eLife.57730⟩ |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.57730 |
| Popis: | Tissue elongation is a central morphogenetic event occurring in all organisms in development1,2. During this process, symmetry of cells and tissues is broken by different mechanisms, such as neighbor exchange3,4, cell elongation5,6 and oriented cell division7. While the phenomenon is known to involve remodeling of adherens junctions4 and acto-myosin4,8 at the molecular level, mesoscopic mechanisms leading to distinct morphogenesis processes are poorly understood. This is partly because inputs from morphogen gradients9 or from neighboring tissues10,11 can affect tissue autonomous self-organization in vivo. It is therefore difficult to disentangle cell intrinsic from externally mediated behaviors. Here we use in vitro experiments and numerical simulations to characterize the spontaneous behavior of a growing cell colony in vitro. We show that in vitro tissue elongation arises from anisotropy in the average cell elongation. This anisotropy sets the direction along which boundary cells migrate radially resulting in a non-isotropic elongation that arises primarily through cell elongation. For colonies submitted to a time periodic stretch, the axis of global symmetry breaking can be imposed by external force, and tissue elongation arises through oriented neighbor exchange. Emergence of radially migrating cells and the interplay between cell elongation and cell rearrangements are confirmed by numerical simulations based on a vertex model. Our results suggest that spontaneous shape deformation is related to the mean orientation of the nematic cell elongation field in the absence of any external input. This provides a framework to explain autonomous tissue elongation and how contributions from different mesoscopic mechanisms can be modulated by external forces. |
| Databáze: | OpenAIRE |
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