Vascular endothelial growth factor transgenic mice exhibit reduced male fertility and placental rejection
| Autor: | R. Poulsom, Roy Bicknell, S. Lui, Lukasz Huminiecki, H.Y. Chan, G. Stamp, A. L. Harris |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Embryology Placenta Gene Expression Endothelial Growth Factors Testicle Kidney Mice chemistry.chemical_compound Testis Promoter Regions Genetic In Situ Hybridization Lymphokines Vascular Endothelial Growth Factors Obstetrics and Gynecology Embryo Vascular endothelial growth factor medicine.anatomical_structure Female Genetically modified mouse medicine.medical_specialty Transgene Enzyme-Linked Immunosorbent Assay Mice Transgenic Biology Proto-Oncogene Proteins Internal medicine In Situ Nick-End Labeling Genetics medicine Animals Humans Receptors Growth Factor Molecular Biology Vascular Endothelial Growth Factor Receptor-1 Mucins Receptor Protein-Tyrosine Kinases Placentation Cell Biology Sperm Mice Inbred C57BL Microscopy Electron Fertility Receptors Vascular Endothelial Growth Factor Endocrinology Reproductive Medicine chemistry Mice Inbred CBA Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 7:255-264 |
| ISSN: | 1460-2407 |
| DOI: | 10.1093/molehr/7.3.255 |
| Popis: | Recent evidence points to the involvement of vascular endothelial growth factor (VEGF) in mammalian reproductive physiology. Transgenic mice expressing VEGF (121 isoform) under the control of the polyepithelial mucin-1 (muc-1) promoter showed a reduction in male fertility due to impaired spermiogenesis, and aberrant placentation leading to preferential rejection of male embryos. A skew in the sex ratio of the litters was seen (three females to two males), independently of whether the transgene was carried by the male or female parent. In-situ hybridization permitted distinction of expression of the human VEGF transgene from endogenous mouse VEGF, and confirmed expression of the transgene in a wide range of epithelial tissues. Expression of the transgene in spermatocytes and in the embryonic portion of placenta is thought to be responsible for the reduced fertility and embryonic resorptions respectively. Males showed either complete sperm maturation arrest or various gradations of partial fertility. Abnormally high or low VEGF in human semen has been reported to be correlated with a lack of pregnancy success following IVF. The muc1-VEGF (121 isoform) transgenic mouse provides an animal model with which to further study this VEGF-induced pathology. |
| Databáze: | OpenAIRE |
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