Detection of the TMPRSS2-ETS fusion gene in prostate carcinomas: retrospective analysis of 55 formalin-fixed and paraffin-embedded samples with clinical data
| Autor: | Cécile Rouzier, Jean Amiel, Jean-François Michiels, Laure Valério, Juliette Haudebourg, Florence Pedeutour, Xavier Carpentier |
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| Přispěvatelé: | Laboratory of Solid Tumors Genetics, Nice University Hospital, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Laboratoire d'Anatomo-Pathologie, Hôpital Pasteur [Nice] (CHU), Laboratoire d'Urologie, Department of Public Health, Hôpital l'Archet |
| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research Oncogene Proteins Fusion Oncogene Proteins MESH: Paraffin Embedding Chromosomal translocation MESH: Formaldehyde law.invention Fusion gene MESH: Aged 80 and over 0302 clinical medicine law Prostate MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Fixatives MESH: Serine Endopeptidases Polymerase chain reaction MESH: Aged Aged 80 and over 0303 health sciences MESH: Middle Aged Paraffin Embedding Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases MESH: Transcription Factors Middle Aged 3. Good health DNA-Binding Proteins medicine.anatomical_structure 030220 oncology & carcinogenesis Adenocarcinoma Adenovirus E1A Proteins PCA3 MESH: Proto-Oncogene Proteins c-ets [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Fixatives 03 medical and health sciences Antigen Formaldehyde Proto-Oncogene Proteins Genetics medicine Humans Molecular Biology Aged Retrospective Studies 030304 developmental biology MESH: Humans Proto-Oncogene Proteins c-ets MESH: Adenocarcinoma Prostatic Neoplasms MESH: Retrospective Studies medicine.disease MESH: Adenovirus E1A Proteins MESH: Male MESH: Proto-Oncogene Proteins [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics MESH: Prostatic Neoplasms Immunology Cancer research Feasibility Studies MESH: Feasibility Studies MESH: DNA-Binding Proteins MESH: Oncogene Proteins Fusion Transcription Factors |
| Zdroj: | Cancer Genetics and Cytogenetics Cancer Genetics and Cytogenetics, Elsevier, 2008, 183 (1), pp.21-7. ⟨10.1016/j.cancergencyto.2008.01.021⟩ |
| ISSN: | 0165-4608 |
| DOI: | 10.1016/j.cancergencyto.2008.01.021 |
| Popis: | The recent identification of fusion genes involving ETS family members in human prostate adenocarcinoma has confirmed the hypothesis that recurrent specific aberrations such as fusion genes may be as frequent in epithelial tumors as they are in leukemias and sarcomas. However, reciprocal translocations with fusion genes are often not detectable in carcinomas by conventional karyotyping because of additional complex chromosomal abnormalities. We retrospectively analyzed a large series of formalin-fixed, paraffin-embedded samples including 55 prostate carcinomas and 11 benign prostate tumors. We identified the fusion gene TMPRSS2-ERG by reverse-transcriptase polymerase chain reaction (RT-PCR) in 40/55 carcinomas (72%). Our study demonstrates that the detection of ETS fusion gene by RT-PCR is feasible on formalin-fixed and paraffin-embedded samples. No significant association between the presence of the fusion gene and any clinical feature, such as preoperative serum prostate-specific antigen (PSA) level (PSA>20 or PSA< or =20), pTNM stage including capsule invasion, seminal vesicle invasion, and lymph nodes metastases, or recurrence was observed in our series. |
| Databáze: | OpenAIRE |
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