Tumor Growth Alters T Cell and Macrophage Production of and Responsiveness to Granulocyte-Macrophage Colony-Stimulating Factor: Partial Dysregulation through Interleukin-10
| Autor: | Carol J. Burger, Klaus D. Elgert, Thomas M. Walker |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty T-Lymphocytes medicine.medical_treatment T cell Immunology Biology Mice Immune system Internal medicine medicine Animals Macrophage Mice Inbred BALB C Macrophages Granulocyte-Macrophage Colony-Stimulating Factor T lymphocyte Molecular biology Interleukin-10 Interleukin 10 Cytokine medicine.anatomical_structure Endocrinology Granulocyte macrophage colony-stimulating factor Sarcoma Experimental CD8 medicine.drug |
| Zdroj: | Cellular Immunology. 154:342-357 |
| ISSN: | 0008-8749 |
| Popis: | Tumor growth induces phenotypic and functional changes among splenic T cells and macrophages (M phi) that contribute to the immunosuppression observed in tumor-bearing hosts (TBH). These changes partly arise through alterations in immune cell production of and responsiveness to cytokines. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important T cell- and M phi-derived cytokine that is produced during normal host immunogenic challenge, but it's involvement during cancer is poorly defined. In contrast, interleukin-10 (IL-10) is an inhibitory cytokine that is produced by immune cells as a deactivation factor. IL-10 can disrupt GM-CSF synthesis and may be associated with tumor-induced changes in cytokine synthesis. We determined if tumor growth alters T-cell and M phi synthesis of and responsiveness to GM-CSF, and if these alterations occur because tumor growth heightens immune cell sensitivity to IL-10. Tumor growth significantly decreased T-cell synthesis of GM-CSF during activation by concanavalin A, and TBH T cells were more susceptible to GM-CSF synthesis inhibition by IL-10 than their normal host (NH) counterparts. This suppression was observed using both unseparated splenic lymphocyte preparations and purified CD4+ and CD8+ T cells. Similarly, TBH M phi (both splenic and peritoneal) produced less GM-CSF than NH M phi during activation by lipopolysaccharide. Tumor growth also altered major histocompatibility complex (MHC) class II- M phi GM-CSF synthesis. TBH M phi were more susceptible to GM-CSF synthesis inhibition by IL-10 than their NH counterparts. Although TBH T cells demonstrate less proliferation than NH T cells during activation, tumor growth did not compromise T-cell responsiveness to GM-CSF. However, tumor growth did increase TBH T-cell susceptibility to inhibition of proliferation by IL-10. Tumor growth suppressed M phi responsiveness to GM-CSF, and IL-10 further decreased M phi responsiveness to GM-CSF. Collectively, these results suggest that T cell and M phi production of and responsiveness to GM-CSF is disrupted during tumor growth, and that TBH T cells and M phi are more susceptible to the suppressor activity of IL-10 than their NH counterparts. |
| Databáze: | OpenAIRE |
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