Rapid Photocrosslinking of Silk Hydrogels with High Cell Density and Enhanced Shape Fidelity
| Autor: | Cui, Xiaolin, Soliman, Bram G, Alcala-Orozco, Cesar R, Li, Jun, Vis, Michelle A M, Santos, Miguel, Wise, Steven G, Levato, Riccardo, Malda, Jos, Woodfield, Tim B F, Rnjak-Kovacina, Jelena, Lim, Khoon S, LS Equine Muscoskeletal Biology, dES RMSC |
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| Přispěvatelé: | LS Equine Muscoskeletal Biology, dES RMSC, Orthopaedic Biomechanics |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
0206 medical engineering Biomedical Engineering Silk Pharmaceutical Science Fibroin Nanotechnology Cell Count 02 engineering and technology macromolecular substances 010402 general chemistry Elastomer SDG 3 – Goede gezondheid en welzijn photo‐polymerization 01 natural sciences Biomaterials SDG 3 - Good Health and Well-being Humans cartilage visible light biofabrication fungi technology industry and agriculture Chemical modification High cell Hydrogels Bond formation 021001 nanoscience & nanotechnology 020601 biomedical engineering 3. Good health 0104 chemical sciences SILK Cartilage Photo-polymerization silk fibroin Self-healing hydrogels 0210 nano-technology Fibroins Chondrogenesis Biofabrication |
| Zdroj: | Advanced healthcare materials, 9(4). John Wiley and Sons Ltd Advanced Healthcare Materials, 9(4):1901667. Wiley |
| ISSN: | 2192-2640 |
| Popis: | Silk fibroin hydrogels crosslinked through di-tyrosine bonds are clear, elastomeric constructs with immense potential in regenerative medicine applications. In this study, demonstrated is a new visible light-mediated photoredox system for di-tyrosine bond formation in silk fibroin that overcomes major limitations of current conventional enzymatic-based crosslinking. This photomediated system rapidly crosslinks silk fibroin (−1) while retaining high cell viability (>80%). The photocrosslinked silk hydrogels present more stable mechanical properties which do not undergo spontaneous transition to stiff, β-sheet-rich networks typically seen for enzymatically crosslinked systems. These hydrogels also support long-term culture of human articular chondrocytes, with excellent cartilage tissue formation. This system also facilitates the first demonstration of biofabrication of silk fibroin constructs in the absence of chemical modification of the protein structure or rheological additives. Cell-laden constructs with complex, ordered, graduated architectures, and high resolution (40 µm) are fabricated using the photocrosslinking system, which cannot be achieved using the enzymatic crosslinking system. Taken together, this work demonstrates the immense potential of a new crosslinking approach for fabrication of elastomeric silk hydrogels with applications in biofabrication and tissue regeneration. |
| Databáze: | OpenAIRE |
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