Structural Characterization of Act c 10.0101 and Pun g 1.0101—Allergens from the Non-Specific Lipid Transfer Protein Family

Autor: Ivana Giangrieco, Maria Antonietta Ciardiello, Maksymilian Chruszcz, Andrea O’Malley, Lisa Tuppo, Anna Gawlicka-Chruszcz, S.S. Pote, Krzysztof Kowal
Rok vydání: 2021
Předmět:
Zdroj: Molecules, Vol 26, Iss 256, p 256 (2021)
Molecules
Volume 26
Issue 2
Molecules (Basel, Online) 26 (2021): 256. doi:10.3390/molecules26020256
info:cnr-pdr/source/autori:O'malley A.; Pote S.; Giangrieco I.; Tuppo L.; Gawlicka-Chruszcz A.; Kowal K.; Ciardiello M.A.; Chruszcz M./titolo:Structural characterization of act c 10.0101 and pun g 1.0101--allergens from the non-specific lipid transfer protein family/doi:10.3390%2Fmolecules26020256/rivista:Molecules (Basel, Online)/anno:2021/pagina_da:256/pagina_a:/intervallo_pagine:256/volume:26
ISSN: 1420-3049
DOI: 10.3390/molecules26020256
Popis: Non-specific lipid transfer proteins (nsLTPs), which belong to the prolamin superfamily, are potent allergens. While the biological role of LTPs is still not well understood, it is known that these proteins bind lipids. Allergen nsLTPs are characterized by significant stability and resistance to digestion. (2) Methods: nsLTPs from gold kiwifruit (Act c 10.0101) and pomegranate (Pun g 1.0101) were isolated from their natural sources and structurally characterized using X-ray crystallography (3) Results: Both proteins crystallized and their crystal structures were determined. The proteins have a very similar overall fold with characteristic compact, mainly &alpha
helical structures. The C-terminal sequence of Act c 10.0101 was updated based on our structural and mass spectrometry analysis. Information on proteins&rsquo
sequences and structures was used to estimate the risk of cross-reactive reactions between Act c 10.0101 or Pun g 1.0101 and other allergens from this family of proteins. (4) Conclusions: Structural studies indicate a conformational flexibility of allergens from the nsLTP family and suggest that immunoglobulin E binding to some surface regions of these allergens may depend on ligand binding. Both Act c 10.0101 and Pun g 1.0101 are likely to be involved in cross-reactive reactions involving other proteins from the nsLTP family.
Databáze: OpenAIRE
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