Combined Short-Term Glucose Starvation and Chemotherapy in 3D Colorectal Cancer Cell Culture Decreases 14-3-3 Family Protein Expression and Phenotypic Response to Therapy
| Autor: | Amanda B. Hummon, Katelyn R. Ludwig, Monica M. Schroll, Emily L. Herring, Gabriel J. LaBonia |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Proteome Cell Survival Colorectal cancer medicine.medical_treatment Antineoplastic Agents Models Biological Article 03 medical and health sciences Structural Biology Spheroids Cellular medicine Humans Viability assay Spectroscopy Starvation Chemotherapy Chemistry Fasting HCT116 Cells medicine.disease Phenotype Glucose 030104 developmental biology 14-3-3 Proteins Cell culture Cancer cell Cancer research medicine.symptom Colorectal Neoplasms |
| Zdroj: | J Am Soc Mass Spectrom |
| ISSN: | 1044-0305 |
| Popis: | Short-term glucose starvation prior to chemotherapy has the potential to preferentially weaken cancer cells, making them more likely to succumb to treatment, while protecting normal cells. In this study, we used 3D cell cultures of colorectal cancer and assessed the effects of short-term glucose starvation and chemotherapy compared to treatment of either individually. We evaluated both phenotypic changes and protein expression levels. Our findings indicate that the combined treatment results in more significant phenotypic responses, including decreased cell viability and clonogenicity. These phenotypic responses can be explained by the decreased expression of LDHA and 14-3-3 family proteins, which were found only in the combined treatment groups. This study indicates that short-term glucose starvation has the potential to increase the efficacy of current cancer treatment regimes. Graphical Abstract ᅟ. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink