Characterization of an OprF-deficient mutant suggests that OprF is an essential protein for Pseudomonas fluorescens strain MF0
| Autor: | Jean-François Burini, Sylvie Chevalier, Marie-Anne Freulet-Marriere, Janine Guespin-Michel, Geert Schoofs, René De Mot, Nicole Orange, Christophe Regeard |
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| Přispěvatelé: | Chercheur indépendant, Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Ctr Microbial & Plant Genet, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Laboratoire de Microbiologie du Froid – Signaux et Micro-Environnement (LMDF-SME) |
| Rok vydání: | 2000 |
| Předmět: |
0303 health sciences
biology 030306 microbiology Pseudomonas Mutant Porins Pseudomonas fluorescens General Medicine biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Microbiology Molecular Weight 03 medical and health sciences Membrane protein Mutation Porin Pseudomonadales Bacterial outer membrane Molecular Biology ComputingMilieux_MISCELLANEOUS Plasmids 030304 developmental biology Pseudomonadaceae |
| Zdroj: | Research in Microbiology Research in Microbiology, Elsevier, 2000, 151 (8), pp.619-627. ⟨10.1016/S0923-2508(00)90128-1⟩ |
| ISSN: | 0923-2508 |
| Popis: | A stable OprF-deficient mutant for Pseudomonas fluorescens strain MF0 was constructed using reverse genetics. This mutant, called MF372, showed a rounded morphology and grew more slowly in minimal medium, but not in rich medium. Contrary to other Pseudomonas strains, the loss of OprF for strain MF0 was accompanied by an altered outer membrane composition. At least three outer membrane proteins were overexpressed, apparently as a consequence of adaptive mutations. The N-terminal sequence of two of them revealed strong similarities with porins of the OprD family from P. aeruginosa. The data presented here shows that OprF may be an essential protein for this P. fluorescens strain. |
| Databáze: | OpenAIRE |
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