Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China

Autor: Lan Yu, Yanjun Fu, Shanshan Su, Chunjiang Li, Yuhang Guo, Kewang Hu, Jisheng Zhang, Xiaoli Zhang, Yu Fu, Yongxin Zhao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
medicine.medical_specialty
China
030106 microbiology
Biology
Fluoroquinolone resistance
beta-Lactamases
law.invention
Microbiology
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Medical microbiology
Plasmid
Bacterial Proteins
law
RNA
Ribosomal
16S
Drug Resistance
Bacterial
Enterobacter cloacae
Pulsed-field gel electrophoresis
medicine
Humans
lcsh:RC109-216
Polymerase chain reaction
Whole genome sequencing
Enterobacteriaceae Infections
CREC
Enterobacter cloacae complex
Metallo-β-lactamase
biochemical phenomena
metabolism
and nutrition
16S ribosomal RNA
bacterial infections and mycoses
Anti-Bacterial Agents
Bacterial Typing Techniques
030104 developmental biology
Infectious Diseases
Carbapenem-Resistant Enterobacteriaceae
Parasitology
Multilocus sequence typing
ST93
Research Article
Fluoroquinolones
Multilocus Sequence Typing
Zdroj: BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-7 (2020)
BMC Infectious Diseases
ISSN: 1471-2334
Popis: Background Enterobacter cloacae complex (ECC) is one of the most common extended-spectrum β-lactamase and carbapenemase-producing pathogen that threatens millions of the elderly and vulnerable sick persons. The objective of this study was to perform the molecular characteristics of the carbapenem-resistant E. cloacae complex (CREC) emerged in Heilongjiang Province of China. Methods Six CREC strains were isolated from the patients with infectious diseases. The identities of ECC isolates were confirmed by sequencing the polymerase chain reaction (PCR) products of 16S rRNA gene. The characterization of the CREC isolates were analyzed by sequencing PCR products of the carbapenemase, ampC and fluoroquinolone resistance genes and performing multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and whole genome sequencing. Results All 6 isolates harbored multiple resistance genes. Of them, 5 carried metallo-β-lactamases and one was blaKPC-2-positive. The levofloxacin and ciprofloxacin-resistant strains had substitutions of gyrA83, gyrA87, and parC80 in the quinolone-resistance determining regions. The MLST analyses revealed that 6 isolates belonged to five sequence types (ST520, ST528, ST1119, ST1120, and ST93) while the PFGE patterns of the isolates fallen into four clusters. The strain ST1120 was found to carry two separated plasmids that encode blaNDM-1 and blaIMP-4. Conclusions Our study, for the first time, identified a CREC strain that co-produces blaNDM-1 and blaIMP-4 in the Northeast China. Our finding emphasizes an urgent need for more intensive surveillance and precaution measures to prevent the CERC spread.
Databáze: OpenAIRE
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