TRIB3 protein denotes a good prognosis in breast cancer patients and is associated with hypoxia sensitivity

Autor: Nicolai Grebenchtchikov, Marloes Wennemers, Fred C.G.J. Sweep, Paul N. Span, Johan Bussink
Rok vydání: 2011
Předmět:
Adult
medicine.medical_treatment
Blotting
Western
Breast Neoplasms
Cell Cycle Proteins
Aetiology
screening and detection [ONCOL 5]
Protein Serine-Threonine Kinases
Real-Time Polymerase Chain Reaction
Risk Assessment
Sensitivity and Specificity
Cohort Studies
Breast cancer
Translational research [ONCOL 3]
Tumor Cells
Cultured
Humans
Medicine
Radiology
Nuclear Medicine and imaging
RNA
Messenger
Hypoxia
Survival rate
Aged
Gene knockdown
Breast neoplasm
business.industry
Biopsy
Needle
TRIB3
Cancer
Hematology
Middle Aged
Hypoxia (medical)
Prognosis
medicine.disease
Immunohistochemistry
Gene Expression Regulation
Neoplastic
Repressor Proteins
Survival Rate
Radiation therapy
Real-time polymerase chain reaction
Oncology
Radiology Nuclear Medicine and imaging
Cancer research
Hormonal regulation Aetiology
screening and detection [IGMD 6]
Female
medicine.symptom
business
Zdroj: Radiotherapy and Oncology, 101, 198-202
Radiotherapy and Oncology, 101, 1, pp. 198-202
ISSN: 0167-8140
DOI: 10.1016/j.radonc.2011.05.057
Popis: Contains fulltext : 97392.pdf (Publisher’s version ) (Closed access) BACKGROUND: Tribbles homolog 3 (TRIB3) is a pseudokinase involved in the regulation of several signaling pathways involved in cell survival and/or cell stress. Here, we determined the correlation between breast cancer prognosis and TRIB3 protein levels and established the role of TRIB3 in cell survival after hypoxia and/or radiotherapy. MATERIAL AND METHODS: TRIB3 mRNA and protein were quantified in a new independent breast cancer patient cohort using QPCR and a new specific avian antibody against TRIB3. In addition, we used siRNA-mediated knockdown of TRIB3 in a colony-forming assay after hypoxia and radiotherapy. RESULTS: TRIB3 mRNA and protein levels did not correlate in breast cancer cell lines or human breast cancer material. We validated our earlier finding that high TRIB3 mRNA denotes a poor prognosis, but found that high TRIB3 protein levels were associated with a good prognosis in breast cancer patients. We also show that knockdown of TRIB3 resulted in an increased survival under hypoxic conditions. CONCLUSION: Whereas mRNA levels of TRIB3 are related with a poor prognosis, TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.
Databáze: OpenAIRE
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