Increase in levels of cyclic AMP during avian limb chondrogenesis in vitro
| Autor: | Michael Solursh, Rebecca S. Reiter, Robert M. Pratt, Patricia Buckley Ahrens |
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| Rok vydání: | 1979 |
| Předmět: |
Cancer Research
medicine.medical_specialty Cell type animal structures Time Factors Mesenchyme Chick Embryo Biology Extracellular matrix Internal medicine medicine Cyclic AMP Animals Wings Animal Molecular Biology Cells Cultured Cartilage Embryo Cell Biology Chondrogenesis In vitro Staining Endocrinology medicine.anatomical_structure Developmental Biology |
| Zdroj: | Differentiation; research in biological diversity. 15(3) |
| ISSN: | 0301-4681 |
| Popis: | In the present study the level of cAMP was measured during in vitro chondrogenesis of wing mesenchyme of stage 24 chick embryos and was found to increase significantly from 6.3 pmol/mg protein at the end of the first day of culture to 9.7 pmol/mg protein on the second day, when chondrogenic expression is first detected by the appearance of an Alcian blue staining extracellular matrix. Nonchondrogenic cultures derived from wings of stage 19 embryos had a lower level of cAMP (4.4 +/- 0.07 pmol/mg protein). The level of cAMP in intact wings was 4.5 +/- 0.4 pmol/mg protein and did not change between stages 19 through 25. The correlatin between increased levels of cAMP and the onset of chondrogenesis is consistent with a role of cAMP in the expression of differentiated functions in chondrocytes, as well as in some other cell types. |
| Databáze: | OpenAIRE |
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