S100A15, an Antimicrobial Protein of the Skin: Regulation by E. coli through Toll-Like Receptor 4
| Autor: | Ronald Wolf, Thomas Ruzicka, Jens U. Wurthner, Sabine Kellermann, Markus Walz, Mohamed Hassan, Richard L. Gallo, V. Lewerenz, Amanda S. Büchau, Gabriela Kukova |
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| Rok vydání: | 2007 |
| Předmět: |
Keratinocytes
Mice Transgenic Human skin Dermatology medicine.disease_cause Biochemistry Antibodies S100 Calcium Binding Protein A7 Microbiology Mice Escherichia coli medicine Animals Humans Receptor Molecular Biology Cells Cultured Toll-like receptor Innate immune system biology Calcium-Binding Proteins Interleukin-8 S100 Proteins Pattern recognition receptor Cell Biology biology.organism_classification Enterobacteriaceae Immunity Innate Mice Inbred C57BL Toll-Like Receptor 4 Gene Expression Regulation TLR4 Female |
| Zdroj: | Journal of Investigative Dermatology. 127:2596-2604 |
| ISSN: | 0022-202X |
| Popis: | E. coli is a Gram-negative bacterium rarely found on human skin. We investigated whether direct interaction of E. coli with keratinocytes might induce an innate immune response through recognition by pattern recognition receptors. The capacity of E. coli to activate innate immune responses and IL-8 induction was investigated. We found that E. coli significantly induced human S100A7 and S100A15 transcript abundance and IL-8 release in cultured primary human keratinocytes. S100A15 is a member of the S100 protein family with previously unknown function. E. coli induced effects could be inhibited by neutralizing Toll-like receptor 4 (TLR4) antibodies, suggesting that E. coli-induced IL-8 and S100A15 expression in human keratinocytes are TLR4 dependent. TLR4-/- mice lacked elevated mS100A15 expression after infection with E. coli in contrast to wild-type mice. In vitro, human S100A15 displayed antimicrobial activity against E. coli. Our findings suggest that E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4. |
| Databáze: | OpenAIRE |
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