Differences in gene expression between natural and artificially induced leaf senescence in barley
| Autor: | Sandra Bartsch, Armin Springer, Georg Acker, Heike Bauerschmitt, Steffen Reinbothe, Christiane Reinbothe |
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| Rok vydání: | 2015 |
| Předmět: |
Chlorophyll
Senescence Physiology Cyclopentanes Plant Science Acetates Biology Genes Plant Thylakoids chemistry.chemical_compound Gene Expression Regulation Plant Gene expression Oxylipins RNA Messenger Plastid Carotenoid Plant Proteins Cell Nucleus chemistry.chemical_classification Methyl jasmonate Singlet Oxygen food and beverages Hordeum beta Carotene Plant Leaves chemistry Biochemistry Oxygenases Hordeum vulgare Thylakoid membrane disassembly Agronomy and Crop Science |
| Zdroj: | Journal of Plant Physiology. 176:180-191 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2015.01.004 |
| Popis: | Senescence is the last step of leaf development in the life span of an annual plant. Senescence can be induced prematurely by treating leaf tissues with jasmonic acid methyl ester (methyl jasmonate, MeJA). During both senescence programmes, drastic changes occur at the biochemical, cellular and ultra-structural levels that were compared here for primary leaves of barley (Hordeum vulgare L.). Our findings indicate that both types of senescence are similar with respect to the morphological changes including the loss of chlorophyll, disintegration of thylakoids, and formation of plastoglobules. However, the time elapsed for reaching senescence completion was different and ranged from 7 to 8 days for artificially senescing, MeJA-treated plants to 7-8 weeks for naturally senescing plants. Pulse-labelling studies along with RNA and protein gel blot analyses showed differential changes in the expression of both plastid and nuclear genes coding for photosynthetic proteins. Several unique messenger products accumulated in naturally and artificially senescing, MeJA-treated leaves. Detailed expression and crosslinking studies revealed that pheophorbide a oxygenase (PAO), a previously implicated key enzyme of chlorophyll breakdown, is most likely not rate-limiting for chlorophyll destruction under both senescence conditions. Metabolite profiling identified differential changes in the composition of carotenoid derivatives and prenyl-lipids to occur in naturally senescing and artificially senescing plants that underscored the differences between both senescence programmes. |
| Databáze: | OpenAIRE |
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