A Shorter Equilibration Period Improves Post-Warming Outcomes after Vitrification and in Straw Dilution of In Vitro-Produced Bovine Embryos
| Autor: | Meritxell Vendrell-Flotats, Joseba Esmoris, Sabino Azcarate, Xabier Mendibil, Iris Martínez-Rodero, Teresa Mogas, C.O. Hidalgo, Tania García-Martínez, Erika Alina Ordóñez-León, Marc Yeste, Manel Lopez-Bejar |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
total cell number
SOX2 cow Apoptosis cryopreservation Cryopreservation 0302 clinical medicine Inner cell mass Vitrification Reproducció assistida lcsh:QH301-705.5 TUNEL Expanded blastocyst 030219 obstetrics & reproductive medicine Bestiar boví -- Embrions apoptosis Embryo 04 agricultural and veterinary sciences Straw inner cell mass Dilution expanded blastocyst embryonic structures Trophectoderm trophectoderm General Agricultural and Biological Sciences animal structures Cattle -- Embryos Biology General Biochemistry Genetics and Molecular Biology Article Andrology 03 medical and health sciences Regulació genètica Genetic regulation General Immunology and Microbiology Hatching Cow 0402 animal and dairy science Total cell number Reproductive technology Expressió gènica 040201 dairy & animal science In vitro lcsh:Biology (General) gene expression Gene expression |
| Zdroj: | Biology, Vol 10, Iss 142, p 142 (2021) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Biology Volume 10 Issue 2 Biology-Basel, 2021, vol. 10, núm. 2, p. 142 Articles publicats (D-B) Martínez Rodero, Iris García Martínez, Tania Ordóñez León, Erika Alina Vendrell i Flotats, Meritxell Hidalgo, Carlos O. Esmoris, Joseba Mendibil, Xabier Azcarate, Sabino López Béjar, Manel Yeste Oliveras, Marc Mogas Amorós, Teresa 2021 A shorter equilibration period improves post-warming outcomes after vitrification and in straw dilution of in vitro-produced bovine embryos Biology-Basel 10 2 142 DUGiDocs – Universitat de Girona instname |
| ISSN: | 2079-7737 |
| Popis: | This study was designed to the optimize vitrification and in-straw warming protocol of in vitro-produced bovine embryos by comparing two different equilibration periods, short equilibrium (SE: 3 min) and long equilibrium (LE: 12 min). Outcomes recorded in vitrified day seven (D7) and day eight (D8) expanded blastocysts were survival and hatching rates, cell counts, apoptosis rate, and gene expression. While survival rates at 3 and 24 h post-warming were reduced (p < 0.05) after vitrification, the hatching rates of D7 embryos vitrified after SE were similar to the rates recorded in fresh non-vitrified blastocysts. The hatching rates of vitrified D8 blastocysts were lower (p < 0.05) than of fresh controls regardless of treatment. Total cell count, and inner cell mass and trophectoderm cell counts were similar in hatched D7 blastocysts vitrified after SE and fresh blastocysts, while vitrified D8 blastocysts yielded lower values regardless of treatment. The apoptosis rate was significantly higher in both treatment groups compared to fresh controls, although rates were lower for SE than LE. No differences emerged in BAX, AQP3, CX43, and IFNτ gene expression between the treatments, whereas a significantly greater abundance of BCL2L1 and SOD1 transcripts was observed in blastocysts vitrified after SE. A shorter equilibration vitrification protocol was found to improve post-warming outcomes and time efficiency after in-straw warming/dilution. |
| Databáze: | OpenAIRE |
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