Modulation of Arabidopsis root growth by specialized triterpenes

Autor: Andrés Ritter, Tom Beeckman, Anne Osbourn, Patricia Fernández-Calvo, Laurens Pauwels, Alain Goossens, Yuechen Bai, Stefania Morales-Herrera, Maria Fransiska Njo, Jacob Pollier, José C. Martins, Keylla U Bicalho, Ancheng C. Huang, Dieter Buyst, Steffen Vanneste, Michal Karady, Karen Ljung
Přispěvatelé: Ghent University, VIB Center for Plant Systems Biology, John Innes Centre, KU Leuven, VIB Center for Microbiology, Universidade Estadual Paulista (Unesp), Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University, Swedish University of Agricultural Sciences, Ghent University Global Campus
Rok vydání: 2021
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1469-8137
0028-646X
DOI: 10.1111/nph.17144
Popis: Made available in DSpace on 2021-06-25T10:50:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 John Innes Foundation Seventh Framework Programme European Regional Development Fund Fonds Wetenschappelijk Onderzoek Plant roots are specialized belowground organs that spatiotemporally shape their development in function of varying soil conditions. This root plasticity relies on intricate molecular networks driven by phytohormones, such as auxin and jasmonate (JA). Loss-of-function of the NOVEL INTERACTOR OF JAZ (NINJA), a core component of the JA signaling pathway, leads to enhanced triterpene biosynthesis, in particular of the thalianol gene cluster, in Arabidopsis thaliana roots. We have investigated the biological role of thalianol and its derivatives by focusing on Thalianol Synthase (THAS) and Thalianol Acyltransferase 2 (THAA2), two thalianol cluster genes that are upregulated in the roots of ninja mutant plants. THAS and THAA2 activity was investigated in yeast, and metabolite and phenotype profiling of thas and thaa2 loss-of-function plants was carried out. THAA2 was shown to be responsible for the acetylation of thalianol and its derivatives, both in yeast and in planta. In addition, THAS and THAA2 activity was shown to modulate root development. Our results indicate that the thalianol pathway is not only controlled by phytohormonal cues, but also may modulate phytohormonal action itself, thereby affecting root development and interaction with the environment. Department of Plant Biotechnology and Bioinformatics Ghent University, Technologiepark 71 VIB Center for Plant Systems Biology, Technologiepark 71 Department of Metabolic Biology John Innes Centre, Norwich Research Park, Colney Lane Laboratory of Molecular Cell Biology KU Leuven, Kasteelpark Arenberg 31 VIB Center for Microbiology, Kasteelpark Arenberg 31 Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) Laboratory of Growth Regulators Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University, Šlechtitelů 27 Department of Organic Chemistry Ghent University Department of Forest Genetics and Plant Physiology Umeå Plant Science Centre Swedish University of Agricultural Sciences Lab of Plant Growth Analysis Ghent University Global Campus Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) Seventh Framework Programme: 613692 TriForC European Regional Development Fund: CZ.02.1.01/0.0/0.0/17_048/0007323 Fonds Wetenschappelijk Onderzoek: G004515N Fonds Wetenschappelijk Onderzoek: G008417N
Databáze: OpenAIRE
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