Differential MicroRNA Expression Profile in Myxomatous Mitral Valve Prolapse and Fibroelastic Deficiency Valves
Autor: | Juan Wang, Chin Cheng Woo, Vitaly Sorokin, Abby S.Y. Wee, Arthur Mark Richards, Edgar Lik Wui Tay, Yei Tsung Chen, Lieng H. Ling, Quek Wei Yong |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Male Pathology Decorin 030204 cardiovascular system & hematology degererative mitral valve disease (DMVD) Extracellular matrix lcsh:Chemistry 0302 clinical medicine Mitral valve Mitral valve prolapse Gene Regulatory Networks 3' Untranslated Regions lcsh:QH301-705.5 Spectroscopy Mitral Valve Prolapse biology microRNA General Medicine Middle Aged fibroelastic deficiency (FED) Computer Science Applications Extracellular Matrix medicine.anatomical_structure Mitral Valve Female ACTA2 medicine.medical_specialty Catalysis Article Inorganic Chemistry 03 medical and health sciences ESM1 medicine Humans Computer Simulation Physical and Theoretical Chemistry Molecular Biology Mitral regurgitation Gene Expression Profiling Organic Chemistry myxomatous mitral valve prolapse (MMVP) medicine.disease MicroRNAs 030104 developmental biology Gene Expression Regulation lcsh:Biology (General) lcsh:QD1-999 biology.protein Desmin |
Zdroj: | International Journal of Molecular Sciences, Vol 17, Iss 5, p 753 (2016) International Journal of Molecular Sciences; Volume 17; Issue 5; Pages: 753 International Journal of Molecular Sciences |
ISSN: | 1422-0067 |
Popis: | Myxomatous mitral valve prolapse (MMVP) and fibroelastic deficiency (FED) are two common variants of degenerative mitral valve disease (DMVD), which is a leading cause of mitral regurgitation worldwide. While pathohistological studies have revealed differences in extracellular matrix content in MMVP and FED, the molecular mechanisms underlying these two disease entities remain to be elucidated. By using surgically removed valvular specimens from MMVP and FED patients that were categorized on the basis of echocardiographic, clinical and operative findings, a cluster of microRNAs that expressed differentially were identified. The expressions of has-miR-500, -3174, -17, -1193, -646, -1273e, -4298, -203, -505, and -939 showed significant differences between MMVP and FED after applying Bonferroni correction (p < 0.002174). The possible involvement of microRNAs in the pathogenesis of DMVD were further suggested by the presences of in silico predicted target sites on a number of genes reported to be involved in extracellular matrix homeostasis and marker genes for cellular composition of mitral valves, including decorin (DCN), aggrecan (ACAN), fibromodulin (FMOD), α actin 2 (ACTA2), extracellular matrix protein 2 (ECM2), desmin (DES), endothelial cell specific molecule 1 (ESM1), and platelet/ endothelial cell adhesion molecule 1 (PECAM1), as well as inverse correlations of selected microRNA and mRNA expression in MMVP and FED groups. Our results provide evidence that distinct molecular mechanisms underlie MMVP and FED. Moreover, the microRNAs identified may be targets for the future development of diagnostic biomarkers and therapeutics. |
Databáze: | OpenAIRE |
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