Platform with biomimetic electrochemical sensors for adiponectin and leptin detection in human serum

Autor: Alina Catrinel Ion, Clara Hortensia Radulescu, Carmen Moldovan, David Dragomir, Bogdan Firtat, Mihaela Savin, Dana Stan, Diana Stan, Carmen Marinela Mihailescu, Marian Ion, George Muscalu, Costin Brasoveanu, Silviu Dinulescu
Rok vydání: 2020
Předmět:
Zdroj: Talanta. 210:120643
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2019.120643
Popis: Two new biomimetic sensors for the detection of adiponectin (A) and leptin (L) through molecularly imprinted polymers (MIPs) onto gold working electrodes (GWEs) were fabricated. Based on electrochemical impedance spectroscopy (EIS) results and cyclic voltammetry (CV) characteristics recorded in the development stages of the fabricated sensors, the sensors were electrochemically optimized and used in an integrated microfluidic platform to detect adiponectin/leptin via conductance signals and non-imprinted electrodes were used as references. To overcome the limitation of the low response signals after template binding non-conductive polyphenol (PP) and poliscopoletin (PS) were used for templates formation. Under optimized experimental conditions the conductance and resistance signals were obtained in the linear range of 0–50 μg ml−1 for A and 1–32 ng∙ml-1 for L with low limits of detection (0.25 μg ml−1 for A and 0.110 ng ml−1 for L). The dedicated platform exhibited an excellent response with great selectivity and stability. Finally, the proposed biomimetic sensors were successfully applied to enable the determination of A and L in human patient's serum with very high accuracy when compared to enzyme-linked immunosorbent assay ELISA reference methods.
Databáze: OpenAIRE
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