Danshen Attenuates Intervertebral Disc Degeneration via Antioxidation in SD Rats
| Autor: | Rongqing Qin, Bing Zhou, Pin Zhou, Shouqian Dai, Xing Zhang, Hongpeng Liu, Chuanliang Hu |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Aging medicine.medical_specialty Article Subject SOD2 Salvia miltiorrhiza Intervertebral Disc Degeneration Biochemistry Antioxidants Rats Sprague-Dawley Glycosaminoglycan Superoxide dismutase chemistry.chemical_compound Internal medicine medicine Animals Aggrecans Intervertebral Disc Aggrecan Glycosaminoglycans Inflammation QH573-671 biology business.industry Intervertebral disc Cell Biology General Medicine Glutathione Malondialdehyde Immunohistochemistry Magnetic Resonance Imaging Extracellular Matrix Rats Disease Models Animal Oxidative Stress Endocrinology medicine.anatomical_structure chemistry biology.protein Female Matrix Metalloproteinase 3 Collagen Cytology business Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2020/6660429 |
| Popis: | Objective. To investigate the effects of Danshen on the imaging and histological parameters, expression levels of ECM-associated proteins and inflammatory factors, and antioxidative activity in the degenerated intervertebral disc (IVD) of SD rats. Methods. Sixty male rats were randomly divided into three groups (control, IDD, and Danshen IDD). Percutaneous needle puncture in Co8-9 intervertebral disc was conducted in all rats of the IDD and Danshen IDD groups to induce intervertebral disc degeneration (IDD). After operation, animals of the Danshen IDD group were administrated with Danshen granules (3 g/kg body weight ) by gavage once a day. Four weeks later, the coccygeal vertebrae were harvested and used for imaging (disc height and MR signal), histological, immunohistochemical, and biochemical [water content, glycosaminoglycans (GAG), superoxide dismutase (SOD2), glutathione (GSH), and malondialdehyde (MDA)] analyses. Results. The puncture induced significant decreased IVD space and MR T2 signal at both 2 and 4 weeks, which were attenuated by Danshen treatment. The disc degeneration in the IDD group (HE and Safranin O-Fast Green histological staining was markedly more serious compared with that in the control group. Four weeks of Danshen treatment significantly alleviated this degeneration compared with the IDD group. Needle puncture resulted in the upregulation of IL-1β and TNF-α, MMP-3, and downregulation of COL2 and aggrecan in the IDD group. However, this change was significantly weakened by Danshen treatment. Significantly lower water and GAG content, as well as the SOD2 and GSH levels, in the IDD group were found compared with those in the control group. However, the above parameters of the Danshen IDD group were significantly higher than those of the IDD group. Danshen treatment significantly decreased the content of MDA which was increased by needle puncture in the IDD group. Conclusion. Danshen can attenuate intervertebral disc degeneration in SD rats by suppressing the oxidation reaction. |
| Databáze: | OpenAIRE |
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