Comparative histological description of the digestive and visual system development of larval chame Dormitator latifrons (Pisces: Eleotridae)
| Autor: | Eva A Medina-Hernández, Vanesa V López-López, Gustavo A Rodríguez M. de O, Cristóbal Román Reyes, Maria Haws, Konrad Dabrowski, Mario A. Galaviz |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gill animal structures food.ingredient Ontogeny Zoology larvae Broodstock Aquatic Science Oceanography 03 medical and health sciences food Dormitator latifrons digestive system Yolk chame larvas ontogenia Mexico sistema digestivo Larva biology México fungi Foregut 04 agricultural and veterinary sciences biology.organism_classification Dormitator 030104 developmental biology ontogeny aquaculture acuicultura 040102 fisheries 0401 agriculture forestry and fisheries |
| Zdroj: | Latin american journal of aquatic research, Volume: 43, Issue: 3, Pages: 484-494, Published: JUL 2015 |
| ISSN: | 0718-560X |
| DOI: | 10.3856/vol43-issue3-fulltext-10 |
| Popis: | Se realizó la descripción del desarrollo de larvas de uno a seis días post-eclosión (dpe), para generar información que ayude a comprender la capacidad alimenticia en etapas tempranas. Las larvas se obtuvieron mediante la inducción hormonal de reproductores, utilizando implantes de análogos sintéticos de GnRH durante la temporada de septiembre-noviembre de 2010. Las muestras fueron incluídas en historesina y teñidas con hematoxilina-eosina. Una vez eclosionada, la larva presenta gran cantidad de vitelo que absorbe gradualmente (1° a 3° dpe). Al 4° dpe, existe una cavidad bucal abierta con estructuras dentales, en este estadio se ha completado el desarrollo ocular, que presenta pigmentación. Se observa la formación de las hendiduras branquiales y se diferencia el intestino en anterior y posterior y se distingue la presencia de vacuolas digestivas en el intestino anterior. En el 5° dpe, se observaron los paquetes de fibras musculares y se distinguió una estructura estomacal. En el 6° dpe, el páncreas, pliegues intestinales y membrana de borde de cepillo son visibles y se observaron partículas de alimento y bacterias dentro del intestino. En base a lo anterior, se concluye que las larvas de D. latifrons han desarrollado completamente el tracto digestivo y órganos asociados que le ayudarán a la búsqueda de alimento, entonces a partir del 4° dpe son capaces de iniciar la alimentación exógena. This study was focused on the morphological description of chame larvae from one to six days post-hatching (dph), in order to generate information that helps understanding their feeding ability in early stages. The larvae were obtained by hormonal induction of a broodstock, using implants of GnRH synthetic analogues, during the spawning season from September to November 2010. The samples were included in historesin, and stained with hematoxylin-eosin. It was described that, once the larva hatched has a large amount of yolk it is gradually absorbed (from 1 to 3 dph). At day four, there is an open oral cavity with dental structures, the eye development is complete and there was pigmentation on it, the gills are observable and the foregut was differentiated in anterior and posterior intestine, showing the presence of digestive vacuoles in the anterior intestine. By five dph the larvae has developed muscle fibers and a structure identified as the stomach. At sixth dph, it is possible to identify the pancreas, the intestinal folds, and the brush border membrane, and there were food particles and bacteria rests in the gut. Based on the above, it is concluded that the larvae of D. latifrons from the fourth dph fully developed organs and systems that help the search of food, so they are capable of starting with exogenous feeding. |
| Databáze: | OpenAIRE |
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