Widespread Protection of RNA Cleavage Sites by a Riboswitch Aptamer that Folds as a Compact Obstacle to Scanning by RNase E
| Autor: | Jamie Richards, Joel G. Belasco |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Riboswitch
0303 health sciences Messenger RNA RNA Folding biology RNase P Aptamer Cell Biology Cleavage (embryo) Article Cell biology Legionella pneumophila 03 medical and health sciences RNA Bacterial 0302 clinical medicine Eukaryotic translation Bacterial Proteins Endoribonucleases biology.protein RNA Cleavage Ribonuclease Molecular Biology 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Mol Cell |
| ISSN: | 1097-4164 |
| Popis: | Riboswitches are thought generally to function by modulating transcription elongation or translation initiation. In rare instances, ligand binding to a riboswitch has been found to alter the rate of RNA degradation by directly stimulating or inhibiting nearby cleavage. Here we show that guanidine-induced pseudoknot formation by the aptamer domain of a guanidine III riboswitch from Legionella pneumophila has a very different effect, stabilizing mRNA by protecting distal cleavage sites en masse from ribonuclease attack. It does so by creating a coaxially base-paired obstacle that impedes scanning from a monophosphorylated 5′ end to those sites by the regulatory endonuclease RNase E. Ligand binding by other riboswitch aptamers peripheral to the path traveled by RNase E does not inhibit distal cleavage. These findings reveal that a riboswitch aptamer can function independently of any overlapping expression platform to regulate gene expression by acting directly to prolong mRNA longevity in response to ligand binding. |
| Databáze: | OpenAIRE |
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