Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
| Autor: | Guang-jian Wang, Meng Gu, Juan Zhou, Feng-fu Guo, Zhong Wang, Yu-Bing Peng, Xiangfei He, Xiang Wang, Wenzhi Li |
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| Rok vydání: | 2015 |
| Předmět: |
Cyclin-dependent kinase 1
Pathology medicine.medical_specialty Cell growth AKT proliferation Cell Cell cycle Biology urologic and male genital diseases medicine.anatomical_structure Oncology DU145 mTOR medicine Cancer research Gene silencing cell cycle prostate cancer (PCa) GOLPH3 Protein kinase B PI3K/AKT/mTOR pathway Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.11228 |
| Popis: | Background: Golgi phosphoprotein 3 (GOLPH3) is a metastasis-associated gene, however its role in cell proliferation of prostate cancer (PCa) has not yet been elucidated. Methods: The level of expression of GOLPH3 and other genes was examined by quantitative real-time PCR (QPCR) and western blot analysis. Furthermore, we performed a comprehensive analysis of the expression of GOLPH3 in PCa using a tissue microarray (TMA) and correlated our findings with pathological parameters of PCa. RNA interference (RNAi) was used to silence the expression of GOLPH3 in PC-3 cells and to measure the effects on proliferation and cell cycle using the CCK-8 assay and flow cytometry. Western blots were also employed to assess AKT-mTOR and cell cycle-related proteins. Results: We showed that the expression of GOLPH3 was located at the trans-Golgi membranes in PCa cells. We found that GOLPH3 was expressed in all PCa cells and was significantly higher in two androgen-independent cell lines, DU145 and PC-3. TMA immunohistochemistry showed that GOLPH3 was positive in 64% of cancer tissue samples compared with 20% in normal and 30% in benign samples (P |
| Databáze: | OpenAIRE |
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