The incorporation of (1-14C) arachidonic acid into the lipids of rabbit renal slices and conversion to prostaglandins E2 and F2
| Autor: | Keith Crowshaw |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Kidney Cortex Renal cortex Arachidonic Acids Biology Fatty Acids Nonesterified In Vitro Techniques Kidney Biochemistry chemistry.chemical_compound Endocrinology Cortex (anatomy) Internal medicine medicine Renal medulla Methods Animals Incubation Phospholipids Carbon Isotopes Kidney Medulla Venoms Hydrolysis Rabbit (nuclear engineering) Lipid Metabolism Lipids medicine.anatomical_structure chemistry Prostaglandins lipids (amino acids peptides and proteins) Arachidonic acid Chromatography Thin Layer Rabbits |
| Zdroj: | Prostaglandins. 3(5) |
| ISSN: | 0090-6980 |
| Popis: | 1. 1. [1- 14 C]Arachidonic acid is taken up by slices of rabbit renal medulla and cortex and incorporated into tissue phospholipids and neutral lipids. Although arachidonic acid is known to be the precursor of PGE 2 and PGF 2α in rabbit renal medulla, no 14 C-labelled prostaglandins were detected in the actual tissue lipids which were extracted from the slices of renal medulla and renal cortex after incubation with [1- 14 C]arachidonic acid. 2. 2. When the incubation fluids from the above experiments were extracted, large yields of 14 C-labelled PGF 2α , PGE 2 and hydroxy acids were detected in the fluids obtained from slices of rabbit renal medulla. In contrast, the incubation fluids obtained from slices of rabbit renal cortex contained no detectable prostaglandins or related metabolites. |
| Databáze: | OpenAIRE |
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