Disruption of Lipid Rafts Interferes with the Interaction of Toxoplasma gondii with Macrophages and Epithelial Cells
| Autor: | Gabriela Veras de Moraes, Thayana A. Cruz, T.C. Paredes-Santos, Márcia Attias, Karla Dias Cruz, Wanderley de Souza |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cell type
Article Subject Cell Survival media_common.quotation_subject Cell lcsh:Medicine Kidney Filipin General Biochemistry Genetics and Molecular Biology Cell Line Mice chemistry.chemical_compound Membrane Microdomains Microscopy Electron Transmission Cell Adhesion medicine Animals Internalization Lipid raft media_common General Immunology and Microbiology biology Macrophages Intracellular parasite beta-Cyclodextrins lcsh:R Toxoplasma gondii Epithelial Cells General Medicine biology.organism_classification Macaca mulatta Sphingolipid Cell biology medicine.anatomical_structure Microscopy Fluorescence chemistry Microscopy Electron Scanning lipids (amino acids peptides and proteins) Toxoplasma Toxoplasmosis Research Article |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | The intracellular parasiteToxoplasma gondiican penetrate any warm-blooded animal cell. Conserved molecular assemblies of host cell plasma membranes should be involved in the parasite-host cell recognition. Lipid rafts are well-conserved membrane microdomains that contain high concentrations of cholesterol, sphingolipids, glycosylphosphatidylinositol, GPI-anchored proteins, and dually acylated proteins such as members of the Src family of tyrosine kinases. Disturbing lipid rafts of mouse peritoneal macrophages and epithelial cells of the lineage LLC-MK2 with methyl-beta cyclodextrin (MβCD) and filipin, which interfere with cholesterol or lidocaine, significantly inhibited internalization ofT. gondiiin both cell types, although adhesion remained unaffected in macrophages and decreased only in LLC-MK2 cells. Scanning and transmission electron microscopy confirmed these observations. Results are discussed in terms of the original role of macrophages as professional phagocytes versus the LLC-MK2 cell lineage originated from kidney epithelial cells. |
| Databáze: | OpenAIRE |
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