3D + time imaging of normal and twin sea urchin embryos for the reconstruction of their cell lineage
| Autor: | Antonio Ortiz, Nadine Peyriéras, Elena Kardash |
|---|---|
| Přispěvatelé: | BioEmergences, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Amro Hamdoun, Kathy R. Foltz |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Image processing Cell lineage Computational biology MESH: Imaging Three-Dimensional Paracentrotus lividus 03 medical and health sciences 3D+time in toto imaging Live cell imaging MESH: Embryonic Development MESH: Animals Interactive visualization 030304 developmental biology 0303 health sciences biology [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior Robustness (evolution) MESH: Embryo Nonmammalian [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences Sea urchin embryo MESH: Cell Lineage biology.organism_classification Embryonic stem cell Early embryogenesis MESH: Image Processing Computer-Assisted MESH: Sea Urchins Twins' resilience MESH: Fertilization Cell lineage reconstruction |
| Zdroj: | Methods in Cell Biology, Echinoderms, Part B Amro Hamdoun, Kathy R. Foltz. Methods in Cell Biology, Echinoderms, Part B, 151, Elsevier, 399-418 chapter 16, 2019, 978-0-12-817072-4. ⟨10.1016/bs.mcb.2019.01.008⟩ Methods in Cell Biology Methods in Cell Biology-Echinoderms, Part B Echinoderms, Part B |
| ISSN: | 0091-679X |
| DOI: | 10.1016/bs.mcb.2019.01.008⟩ |
| Popis: | International audience; The Mediterranean sea urchin, Paracentrotus lividus, has been a powerful model to study embryonic development since the late 1800s. As a model, it has the advantage of having external fertilization, it can easily be manipulated experimentally, and it has semi-transparent embryonic stages, which makes it ideal for live imaging. Embryogenesis is a highly dynamic process with intrinsic variability. The reconstruction of cell dynamics and an assessment of such variability from in vivo observations has proven to be a challenge. Here, we provide an innovative methodology for manipulation and immobilization of embryos and their long-term 3D+time imaging. We then describe the twinning procedure that allows us to assess the variability and robustness of developmental processes. We demonstrate the reconstruction of cell lineages based on automated image processing and cell tracking using the BioEmergences workflow as well as the use of interactive visualization tools (Mov-IT software) for lineage validation, correction and analysis. |
| Databáze: | OpenAIRE |
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