Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss
Autor: | D. Kacy Cullen, Halimulati Kaisaier, Zarina S. Ali, Kevin D. Browne, Foteini Mourkioti, Melanie C Hilman, Franco A Laimo, Carlos A. Aguilar, Suradip Das, Joseph C. Maggiore |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Male
0301 basic medicine Cell Survival medicine.medical_treatment Neuromuscular Junction Medicine (miscellaneous) Cell Count Skeletal Myocytes Revascularization Article General Biochemistry Genetics and Molecular Biology Neuromuscular junction Mice Rats Nude 03 medical and health sciences 0302 clinical medicine medicine Animals Regeneration Implants Muscle Skeletal lcsh:QH301-705.5 Motor Neurons Muscle Cells Tissue engineered Tissue Engineering Tissue Scaffolds Muscle loss business.industry Regeneration (biology) Skeletal muscle Functional recovery Rats Cell biology Muscular Atrophy 030104 developmental biology medicine.anatomical_structure Cellular Microenvironment lcsh:Biology (General) General Agricultural and Biological Sciences business 030217 neurology & neurosurgery |
Zdroj: | Communications Biology, Vol 3, Iss 1, Pp 1-14 (2020) Communications Biology |
ISSN: | 2399-3642 |
Popis: | Volumetric muscle loss (VML) is the traumatic or surgical loss of skeletal muscle beyond the inherent regenerative capacity of the body, generally leading to severe functional deficit. Formation of appropriate somato-motor innervations remains one of the biggest challenges for both autologous grafts as well as tissue-engineered muscle constructs. We aim to address this challenge by developing pre-innervated tissue-engineered muscle comprised of long aligned networks of spinal motor neurons and skeletal myocytes on aligned nanofibrous scaffolds. Motor neurons led to enhanced differentiation and maturation of skeletal myocytes in vitro. These pre-innervated tissue-engineered muscle constructs when implanted in a rat VML model significantly increased satellite cell density, neuromuscular junction maintenance, graft revascularization, and muscle volume over three weeks as compared to myocyte-only constructs and nanofiber scaffolds alone. These pro-regenerative effects may enhance functional neuromuscular regeneration following VML, thereby improving the levels of functional recovery following these devastating injuries. Das et al. create a bioengineered construct combining nerve and muscle cells on a biomimetic scaffold to improve recovery from volumetric muscle loss (VML). When implanted into a rat VML model, they report an increase in in vitro parameters of muscle fiber development and formation of neuromuscular junctions, which support in vivo observations of improved implanted cell survival and vascularization. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |