Identification of Conidiogenesis-Associated Genes in Colletotrichum gloeosporioides by Agrobacterium tumefaciens-Mediated Transformation
| Autor: | Zhang Junxiang, Zhou Zongshan, Jianyuan Wu, Xu Chengnan, Fu-mei Chi, Na Wang, Ji Zhirui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetic Vectors Conidiation Biology Applied Microbiology and Biotechnology Microbiology Conidium Fungal Proteins 03 medical and health sciences Transformation Genetic Sporogenesis Colletotrichum Plant Diseases Fungal protein fungi Fungal genetics General Medicine Agrobacterium tumefaciens Spores Fungal biology.organism_classification Mutagenesis Insertional Transformation (genetics) 030104 developmental biology |
| Zdroj: | Current Microbiology. 73:802-810 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-016-1131-8 |
| Popis: | The Colletotrichum gloeosporioides is one of the most significant pathogens leading to huge economic losses. To infect plants and cause disease dissemination, the fungus elaborates to produce asexual spores called conidia, which are long-lived and highly resistant to environmental stresses. Here, we report a large-scale, systematic genome-wide screening of conidiogenesis-associated genes via conidiation assays, and high-efficiency TAIL-PCRs. Of 10,210 independent transformants tested, 59 mutants exhibited significant variation in conidial production. The T-DNA right flanking sequences of 11 conidiation-related transformants were further identified, and the obtained sequences were aligned to the genome sequence to uncover the novel loci of sporogenesis. When considering together, this study provided a large number of conidial production-variation mutants and the conidiation-related genes, which will be a valuable resource for characterizing the molecular mechanisms of conidial formation in the fungus. |
| Databáze: | OpenAIRE |
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