MMP1-1607 polymorphism increases the risk for periapical lesion development through the upregulation MMP-1 expression in association with pro-inflammatory milieu elements
| Autor: | Ariadne Letra, Carolina Favaro Francisconi, Camile Bermejo Andreo, Renato Menezes Silva, Elcia Maria Varize Silveira, Gustavo Pompermaier Garlet, Ana Paula Favaro Trombone, Angélica Cristina Fonseca, Franco Cavalla |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Genetic Markers Male 0301 basic medicine Pathology medicine.medical_specialty Adolescent Genotype MMP1 Periapical Granuloma Biology Real-Time Polymerase Chain Reaction Statistics Nonparametric CITOCINAS Young Adult 03 medical and health sciences 0302 clinical medicine Periapical granuloma Genetic Reference Values Risk Factors medicine Humans Periapical Diseases Polymorphism General Dentistry Genotyping Genetic Association Studies Inflammation Polymorphism Genetic Case-control study FOXP3 Original Articles 030206 dentistry Middle Aged Up-Regulation lcsh:RK1-715 Matrix metalloproteinases 030104 developmental biology Real-time polymerase chain reaction lcsh:Dentistry Case-Control Studies Regression Analysis Cytokines Female Matrix Metalloproteinase 1 Periapical diseases |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science v.24 n.4 2016 Journal of applied oral science Journal of Applied Oral Science Journal of Applied Oral Science, Vol 24, Iss 4, Pp 366-375 Journal of Applied Oral Science, Volume: 24, Issue: 4, Pages: 366-375, Published: AUG 2016 |
| ISSN: | 1678-7757 |
| Popis: | Increased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective In this study, we evaluated the association between the MMP1-1607 polymorphism (rs1799750) and pro-inflammatory milieu elements with MMP-1 mRNA levels in vivo. Material and Methods MMP1-1607 SNP and the mRNA levels of MMP-1, TNF-a, IFN-g, IL-17A, IL-21, IL-10, IL-4, IL-9, and FOXp3 were determined via RealTimePCR in DNA/RNA samples from patients presenting periapical granulomas (N=111, for both genotyping and expression analysis) and control subjects (N=214 for genotyping and N=26 for expression analysis). The Shapiro-Wilk, Fisher, Pearson, Chi-square ordinal least squares regression tests were used for data analysis (p |
| Databáze: | OpenAIRE |
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