Inhibition of 5alpha-reductase activity in SZ95 sebocytes and HaCaT keratinocytes in vitro
| Autor: | Seiffert K, Christos C. Zouboulis, Seltmann H, Martin Fritsch |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Keratinocytes
medicine.medical_specialty Cholestenone 5 alpha-Reductase Endocrinology Diabetes and Metabolism Clinical Biochemistry Biology Biochemistry chemistry.chemical_compound Sebaceous Glands Endocrinology Internal medicine medicine Androgen Receptor Antagonists Humans Testosterone Acne Cells Cultured Cell Proliferation Cell growth Biochemistry (medical) Finasteride Cyproterone acetate General Medicine medicine.disease Androgen receptor HaCaT Turosteride chemistry Azasteroids Protein Biosynthesis Androgens |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 39(2) |
| ISSN: | 0018-5043 |
| Popis: | Inhibition of 5alpha-reductase type 1 has been considered to be a promising target for treatment of androgen-dependent skin disorders, however, currently published clinical results on acne treatment are rather disappointing. In this study, the influence of selective inhibitors of 5alpha-reductase on testosterone metabolism within SZ95 sebocytes and HaCaT keratinocytes IN VITRO was investigated. In both cell types, the isotype 1 inhibitor MK386 completely inhibited the conversion of testosterone to 5alpha-dihydrotestosterone in concentrations higher than 10 (-9) M. Inhibitors of the isotype 2 such as finasteride, dihydrofinasteride, and turosteride, were >100-fold less active, while, as expected, androgen receptor inhibitors did not affect the 5alpha-reductase activity. MK386, but not finasteride, reduced testosterone-stimulated proliferation and slightly reduced the testosterone-induced increase in the amount of SZ95 sebocyte proteins. The androgen receptor inhibitor cyproterone acetate exhibited no effect on testosterone-induced proliferation, but inhibited the 5alpha-dihydrotestosterone-induced sebocyte proliferation. Our experimental findings and the existing clinical results indicate that the inhibition of 5alpha-reductase activity alone may be insufficient to reduce overall sebocyte activity and improve acne lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|