High genetic and epigenetic stability in Coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by AFLP, MSAP and the phenotypic variation rate
| Autor: | Benoît Bertrand, June Simpson, Sylvain Santoni, Frederic Georget, Juan Carlos Herrera, Eveline Dechamp, Hervé Etienne, Gloria Camayo, Alberto Cenci, Philippe Lashermes, Roberto Bobadilla Landey |
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| Přispěvatelé: | Résistance des plantes aux bio-agresseurs (UMR RPB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 2 - Sciences et Techniques (UM2), Centro Nacional de Investigaciones de Café (CENICAFE), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Department of Plant Genetic Engineering, Instituto Politecnico Nacional [Mexico] (IPN), Mexican Government through Consejo Nacional de Ciencia y Tecnologia (CONACyT) program [CVU:1623391], PCP France-Mexico, CIRAD funds, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Etienne, Hervé, Université Montpellier 2 - Sciences et Techniques (UM2)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Somatic embryogenesis [SDV]Life Sciences [q-bio] Plant embryogenesis Phénotype lcsh:Medicine Coffea Plant Science 01 natural sciences Somaclonal variation F30 - Génétique et amélioration des plantes Epigenesis Genetic Tissue Culture Techniques Variation clonale Embryogénèse somatique Morphogenesis Plant Genomics Amplified Fragment Length Polymorphism Analysis lcsh:Science Génétique 2. Zero hunger Genetics Plant Growth and Development 0303 health sciences Multidisciplinary Karyotype Agriculture Cell Differentiation Coffea arabica Genomics Plants Phenotype Seeds Culture d'embryon Research Article Biotechnology Histology Mitosis Biology Chromosomes Plant 03 medical and health sciences Genetic variation Botany Régénération in vitro 030304 developmental biology Hybrid Ploidies Polymorphism Genetic Tissue Engineering lcsh:R Chromosome Genetic Variation DNA Methylation Hybridization Genetic Amplified fragment length polymorphism lcsh:Q Plant Biotechnology Stabilité génétique Organism Development 010606 plant biology & botany Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (2), ⟨10.1371/journal.pone.0056372⟩ PloS One Plos One 2 (8), . (2013) PLoS ONE, Vol 8, Iss 2, p e56372 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0056372⟩ |
| Popis: | Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagation of C. arabica hybrids, suspension protocols based on low 2,4-D concentrations and short proliferation periods were developed. As mechanisms leading to somaclonal variation are often complex, the phenotypic, genetic and epigenetic changes were jointly assessed so as to accurately evaluate the conformity of suspension-derived plants. The effects of embryogenic suspensions and secondary embryogenesis, used as proliferation systems, on the genetic conformity of somatic embryogenesis-derived plants (emblings) were assessed in two hybrids. When applied over a 6 month period, both systems ensured very low somaclonal variation rates, as observed through massive phenotypic observations in field plots (0.74% from 200 000 plant). Molecular AFLP and MSAP analyses performed on 145 three year-old emblings showed that polymorphism between mother plants and emblings was extremely low, i.e. ranges of 0-0.003% and 0.07-0.18% respectively, with no significant difference between the proliferation systems for the two hybrids. No embling was found to cumulate more than three methylation polymorphisms. No relation was established between the variant phenotype (27 variants studied) and a particular MSAP pattern. Chromosome counting showed that 7 of the 11 variant emblings analyzed were characterized by the loss of 1-3 chromosomes. This work showed that both embryogenic suspensions and secondary embryogenesis are reliable for true-to-type propagation of elite material. Molecular analyses revealed that genetic and epigenetic alterations are particularly limited during coffee somatic embryogenesis. The main change in most of the rare phenotypic variants was aneuploidy, indicating that mitotic aberrations play a major role in somaclonal variation in coffee. |
| Databáze: | OpenAIRE |
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