Pukyongia salina gen. nov., sp. nov., a novel genus in the family Flavobacteriaceae
| Autor: | Yeon Hee Jang, Seong-Jin Kim, Young-Sam Kim, Kyoung-Ho Kim |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Lineage (evolution) Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Genus RNA Ribosomal 16S Republic of Korea Seawater Gene Phospholipids Phylogeny 030304 developmental biology 0303 health sciences Phylogenetic tree biology Strain (chemistry) 030306 microbiology Fatty Acids Nucleic Acid Hybridization Vitamin K 2 General Medicine Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Flavobacteriaceae Bacterial Typing Techniques Bacteria |
| Zdroj: | Journal of microbiology (Seoul, Korea). 58(6) |
| ISSN: | 1976-3794 |
| Popis: | A Gram-negative aerobic bacterium, designated RR4-38T, was isolated from a biofilter in a seawater recirculating aqua-culture system (RAS) in Busan, South Korea. The bacteria were irregular, short, rod-shaped, non-motile, oxidase-positive, and catalase-negative. Growth of the strain RR4-38T was observed at 15-35·C (optimum, 25-30·C), pH 5.5-9.5 (optimum, pH 8.0), and in the presence of 0-5% (w/v) NaCl (optimum, 3%). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain RR4-38T formed a distinct lineage with close genera Ulvibacter (≤ 95.01% 16S rRNA gene sequence similarity), Aureitalea (94.74%), Aureisphaera (≤ 93.27%), and Jejudonia (93.07%) that all belong to the family Flavobacteriaceae. Whole-genome sequence comparison revealed that the ANI (average nucleotide identity) and digital DDH (DNA-DNA hybridization) values between strain RR4-38T and the two closest strains, Ulvibacter antarcticus DSM 23424T and Aureitalea marina S1-66T, were 68.96-69.88% and 17.4-19%, respectively. The genome analysis revealed that the strain might be involved in biodegradation of organic debris produced by farmed fish in aquaculture systems. The predominant respiratory quinone was menaquinone MK-6 and the major cellular fatty acids were iso-C15:0 (26.5%), iso-C17:0 3-OH (16.4%), iso-C15:1 G (15%), and iso-C16:0 3-OH (9.6%). The major cellular polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipids, and glycolipids. Based on phenotypic, chemotaxonomic, and phylogenetic features, strain RR4-38t represents a novel genus and species in the family Flavobacteriaceae, for which the name Pukyongia salina gen. nov., sp. nov. is proposed. The type strain is RR4-38T (= KCTC 52651T = DSM 108068T). |
| Databáze: | OpenAIRE |
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