The CT-Element of The C-Myc Gene Does Not Predispose to Chromosomal Breakpoints in Burkitt'S Lymphoma
| Autor: | Achim Weber, Marina I. Gutierrez, David Levens |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Chromosome engineering Molecular Sequence Data Genes myc Chromosomal translocation Biology Polymerase Chain Reaction lcsh:RC254-282 Translocation Genetic Pathology and Forensic Medicine chemistry.chemical_compound Cell Line Tumor Tumor Cells Cultured medicine Humans lcsh:QH573-671 Gene Genetics Base Composition DNA-conformation Burkitt's lymphoma Base Sequence lcsh:Cytology Chromosomal fragile site LM-PCR Breakpoint chromosomal breakpoints Chromosome Breakage DNA Cell Biology General Medicine medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Burkitt Lymphoma Lymphoma Blotting Southern Immunoglobulin M chemistry Nucleic Acid Conformation Molecular Medicine Other |
| Zdroj: | Cellular Oncology, Vol 28, Iss 1-2, Pp 31-35 (2006) Cellular Oncology : the Official Journal of the International Society for Cellular Oncology |
| ISSN: | 1875-8606 1570-5870 |
| Popis: | Background: Chromosomal translocations are causally related to the development of many tumors. In Burkitt's lymphoma, abnormalities involving the c-myc gene are essential. The CT-element of the c-myc promoter adopts non-B-conformation in vivo and in vitro, and therefore provides a potential fragile site. Methods: We have developed a LM-PCR-based approach to test if chromosomal breakpoints indeed cluster in this region. Results: Amplifying both, wild-type as well as the translocated c-myc gene by LM-PCR, it was shown that chromosomal breakpoints did not cluster within the CT-element. Conclusions: Therefore, the CT-element is not especially susceptible to the formation of breakpoints leading to chromosomal translocations in Burkitt's lymphoma. |
| Databáze: | OpenAIRE |
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