Yeast-to-hypha transition of schizosaccharomyces japonicus in response to environmental stimuli
| Autor: | Kinnaer, Cassandre, Dudin, Omaya, Martin, Sophie G., Lew, Daniel J. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell Physiology
Cell division Hypha Cèl·lules Hyphae Hyphal tip Biology Microtubules Fungal Proteins 03 medical and health sciences Schizosaccharomyces Cell Cycle/genetics Cell Division/genetics Cytoskeleton/physiology Fungal Proteins/genetics Fungi/cytology Hyphae/cytology Microtubules/physiology Schizosaccharomyces/cytology Schizosaccharomyces/metabolism Vacuoles/physiology Plantes -- Cèl·lules i teixits Molecular Biology Cytoskeleton 030304 developmental biology 0303 health sciences Fungal protein 030306 microbiology Cell Cycle fungi Spitzenkörper Fungi Articles Cell Biology biology.organism_classification Yeast Cell biology Vacuoles Fongs -- Espècimens tipus Cell Division Dimorphic fungus |
| Zdroj: | Molecular biology of the cell, vol. 30, no. 8, pp. 975-991 Molecular Biology of the Cell |
| Popis: | Many fungal species are dimorphic, exhibiting both unicellular yeast-like and filamentous forms. Schizosaccharomyces japonicus, a member of the fission yeast clade, is one such dimorphic fungus. Here, we first identify fruit extracts as natural, stress-free, starvation-independent inducers of filamentation, which we use to describe the properties of the dimorphic switch. During the yeast-to-hypha transition, the cell evolves from a bipolar to a unipolar system with 10-fold accelerated polarized growth but constant width, vacuoles segregated to the nongrowing half of the cell, and hyper-lengthening of the cell. We demonstrate unusual features of S. japonicus hyphae: these cells lack a Spitzenkörper, a vesicle distribution center at the hyphal tip, but display more rapid cytoskeleton-based transport than the yeast form, with actin cables being essential for the transition. S. japonicus hyphae also remain mononuclear and undergo complete cell divisions, which are highly asymmetric: one daughter cell inherits the vacuole, the other the growing tip. We show that these elongated cells scale their nuclear size, spindle length, and elongation rates, but display altered division size controls. This establishes S. japonicus as a unique system that switches between symmetric and asymmetric modes of growth and division. |
| Databáze: | OpenAIRE |
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