Enhanced cyclooxygenase-2 expression in sporadic and familial adenomatous polyposis of the human colon
| Autor: | B. M. Woerner, J. L. Masferrer, R. Soslow, K. N. M. Khan, A. T. Koki |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adenomatous polyposis coli medicine.disease_cause Isozyme Gene Expression Regulation Enzymologic Familial adenomatous polyposis Prostaglandin-endoperoxide synthase 2 Pathogenesis chemistry.chemical_compound Medicine Humans Aged Aged 80 and over biology business.industry Gastroenterology Membrane Proteins Middle Aged medicine.disease Immunohistochemistry Up-Regulation Gene Expression Regulation Neoplastic Isoenzymes chemistry Adenomatous Polyposis Coli Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Colonic Neoplasms biology.protein Cyclooxygenase 1 Female Cyclooxygenase business Carcinogenesis |
| Zdroj: | Scandinavian journal of gastroenterology. 36(8) |
| ISSN: | 0036-5521 |
| Popis: | The cyclooxygenase (COX) enzymes exist in two related but unique isoforms (COX-1 and COX-2) and catalyze the formation of prostaglandins (PGs). COX-1 is constitutively expressed, and is responsible for the synthesis of PGs necessary for gastroprotection and normal renal function. The COX-2 isoform is important in a variety of pathophysiological conditions such as inflammation and tumorigenesis. Numerous studies report that regular use of non-steroidal anti-inflammatory drugs (NSAIDs) can decrease the incidence of some tumor types, including gastrointestinal polyposis.In this study, we evaluated COX-1 and COX-2 expression in 30 polyps collected from 10 patients with familial adenomatous polyposis (FAP) and in 18 polyps collected from 18 patients with sporadic adenomatous polyposis (SAP) using COX-1 or COX-2 isoform-specific antibodies. All tissues were formalin-fixed and paraffin-embedded. Immunoreactivity was detected using tyramide signal amplification and evaluated utilizing an immunohistochemical scoring system.COX-2 was minimally detected in the distant non-neoplastic epithelium, which also served as an internal negative control. In comparison, all polyps collected from SAP or FAP patients overexpressed COX-2 in the neoplastic epithelial cells (Por = 0.002). Additionally, pronounced COX-2 expression was observed in the stromal cells underlying and adjacent to adenomatous lesions. COX-1 immunoreactivity was weak to mild throughout each tissue evaluated and did not change in the neoplastic or stromal cells of the polyps.COX-2 expression is upregulated in the adenomatous epithelium of SAP and FAP, while the COX-1 isoform appears to be constitutively expressed at low levels in both neoplastic and non-neoplastic regions. The differential expression of COX-1 and COX-2 in these neoplasms suggests that COX-2 rather than COX-1 may play a role in adenoma formation and/or growth in cases of SAP and FAP in humans. |
| Databáze: | OpenAIRE |
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