Replacement of the deoxycytidine residues inRhizobiumbacteriophage RL38JI DNA
| Autor: | Stanley Hattman, David Swinton, Vicky Buchanan-Wollaston, John E. Beringer, Rolf Benzinger |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Deoxyribonucleosides
Biophysics Deoxycytidine Biochemistry Bacteriophage 03 medical and health sciences chemistry.chemical_compound Transduction (genetics) Structural Biology Genetics Bacteriophages Molecular Biology 030304 developmental biology 0303 health sciences Nuclease Base Sequence biology 030306 microbiology Spectrum Analysis Dado Rhizobium phage DNA modificaton Deoxycytidine DNA Restriction Enzymes Cell Biology biology.organism_classification Molecular biology Restriction enzyme chemistry DNA Viral biology.protein DNA Rhizobium Micrococcal nuclease |
| Zdroj: | FEBS Letters. 184:294-298 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(85)80625-6 |
| Popis: | Rhizobium phage RL38JI DNA is resistant to cleavage by a variety of restriction endonucleases, and is only partially sensitive to digestion by pancreatic DNase I or by micrococcal nuclease. We have found that a mixture of DNase I, P1 nuclease, and bacterial alkaline phosphatase will quantitatively digest RL38JI DNA to deoxyribonucleosides. HPLC analysis revealed that dCyd is nearly totally absent among these digestion products, while dGuo, dAdo, and Thd are readily detected. Three additional peaks are always present; their retention properties correspond to no known modified deoxyribonucleosides. Thus it appears that dCyd is replaced in phage RL38JI DNA by as many as 3 different modified residues. |
| Databáze: | OpenAIRE |
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