A sensitive retroviral pseudotype assay for influenza H5N1-neutralizing antibodies
| Autor: | Carolyn Nicolson, Ruth J. Manvell, Katja Hoschler, Vo Minh Hien, Yasuhiro Takeuchi, Robin A. Weiss, Diane Major, Nigel J. Temperton, Menno De Jong, Maria Zambon, Do Quang Ha |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Epidemiology serology Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity Poultry Neutralization Serology 03 medical and health sciences Orthomyxoviridae Infections Neutralization Tests Influenza Human Influenza A virus medicine Animals Humans Neutralizing antibody 030304 developmental biology viral neutralization 0303 health sciences Sheep Influenza A Virus H5N1 Subtype 030306 microbiology Ferrets Public Health Environmental and Occupational Health Original Articles H5N1 Hemagglutination Inhibition Tests Virology Influenza A virus subtype H5N1 3. Good health Titer pseudotypes Retroviridae Infectious Diseases Influenza in Birds biology.protein Female Antibody influenza |
| Zdroj: | Influenza and other respiratory viruses, 1(3), 105-112. Wiley-Blackwell Influenza and Other Respiratory Viruses |
| ISSN: | 1750-2659 |
| Popis: | Background The World Health Organisation (WHO) recommended the development of simple, safe, sensitive and specific neutralization assays for avian influenza antibodies. We have used retroviral pseudotypes bearing influenza H5 hemagglutinin (HA) as safe, surrogate viruses for influenza neutralization assays which can be carried out at Biosafety Level 2. Results Using our assay, sera from patients who had recovered from infection with influenza H5N1, and sera from animals experimentally immunized or infected with H5 tested positive for the presence of neutralizing antibodies to H5N1. Pseudotype neutralizing antibody titers were compared with titers obtained by hemagglutinin inhibition (HI) assays and microneutralization (MN) assays using live virus, and showed a high degree of correlation, sensitivity and specificity. Conclusions The pseudotype neutralization assay is as sensitive as horse erythrocyte HI and MN for the detection of antibodies to H5N1. It is safer, and can be applied in a high-throughput format for human and animal surveillance and for the evaluation of vaccines. |
| Databáze: | OpenAIRE |
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