Serologic analysis of white-tailed deer sera for antibodies to Borrelia burgdorferi by enzyme-linked immunosorbent assay and western immunoblotting
| Autor: | R G McLean, D F Neitzel, Russell C. Johnson, James S. Gill |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Microbiology (medical) Minnesota Blotting Western Enzyme-Linked Immunosorbent Assay Spirochaetaceae Disease Vectors Tick Serology Lyme disease Borrelia burgdorferi Group Antigen medicine Animals Borrelia burgdorferi Lyme Disease biology Deer biology.organism_classification medicine.disease Antibodies Bacterial Virology Evaluation Studies as Topic Humoral immunity biology.protein Female Antibody Research Article |
| Zdroj: | Journal of Clinical Microbiology. 31:318-322 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.31.2.318-322.1993 |
| Popis: | White-tailed deer serum samples were collected in the Minneapolis-St. Paul, Minn., metropolitan area during the fall and winter months from 1989 to 1992 and analyzed for antibodies to Borrelia burgdorferi, the etiologic agent of Lyme borreliosis. Ninety-eight percent of the serum samples were collected from regions where currently the vector tick, Ixodes dammini, is nonexistent. Antibodies to B. burgdorferi were detected in 2.2% of 508 samples by enzyme-linked immunosorbent assay, and their presence was confirmed by Western immunoblot analysis. Western immunoblotting yielded mean numbers of reactive bands of 0.1 and 6.0 for samples that were negative and positive for antibodies by enzyme-linked immunosorbent assay, respectively. The molecular weights of the antigens in many of the reactive bands from positive samples were similar to the molecular weights of antigens reactive with samples from humans with Lyme borreliosis. An antibody response to the major outer surface proteins A and B was not detected. Serologic analysis of deer sera may provide a valuable method for surveillance programs designed to monitor the spread of B. burgdorferi in nature. |
| Databáze: | OpenAIRE |
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