Mapping Isomeric Peptides Derived from Biopharmaceuticals Using High-Resolution Ion Mobility Mass Spectrometry
| Autor: | Martin Palmer, Peter Kresten Nielsen, Keith Richardson, Jakub Ujma, Kevin Giles, Nick Tomczyk, Kim F. Haselmann |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Biological Products Chromatography Chemistry Ion-mobility spectrometry High resolution Peptide Mass spectrometry Mass Spectrometry Analytical Chemistry Biopharmaceutical industry Ion Mobility Spectrometry Posttranslational modification Isobaric process Peptides Chromatography Liquid |
| Zdroj: | Analytical Chemistry. 93:16379-16384 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | The identification and localization of isomeric peptide modifications is a critical requirement of the biopharmaceutical industry. Despite the ability of liquid chromatography-mass spectrometry to identify many of the common post translational modifications, the identification of isobaric or racemized peptides is confounded by modern mass spectrometry-based techniques. Here, we present a novel approach combining liquid chromatography with a high-resolution ion mobility mass spectrometry system to differentiate peptide and peptide fragments based upon their mobility and mass. |
| Databáze: | OpenAIRE |
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