A dicentric chromosome of Drosophila melanogaster showing alternate centromere inactivation
| Autor: | Isabel Molina, Ana Losada, Pedro Ripoll, Alfredo Villasante, Marta Agudo, José P. Abad |
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| Rok vydání: | 2000 |
| Předmět: |
Genetics
Yeast artificial chromosome Derivative chromosome Centromere Fluorescent Antibody Technique Biology Protein Serine-Threonine Kinases Telomere Dicentric chromosome Genomic Imprinting Chromosome 4 Drosophila melanogaster Heterochromatin Y Chromosome Cytogenetic Analysis Animals Chromosome 21 Chromosomes Artificial Yeast Protein Kinases Genetics (clinical) In Situ Hybridization Fluorescence Chromosomal inversion Satellite chromosome |
| Zdroj: | Scopus-Elsevier CIÊNCIAVITAE |
| ISSN: | 0009-5915 |
| Popis: | Dicentric chromosomes are rarely found, because they interfere with normal cell division causing chromosome instability. By in situ hybridization of region-specific heterochromatic yeast artificial chromosomes we have found that the artificially generated C(1)A chromosome of Drosophila melanogaster has two potential centromeres: one carries all the sequences of the centromere of the Y chromosome and the other carries only a part of the Y centromeric region that is rich in telomere-related sequences. Immunostaining with anti-Bub1 (a kinetochore-specific marker) shows that, in spite of the differences in sequence, both centromeres can be active although as a rule only one at a time. In a small fraction of the chromosomes centromere inactivation is incomplete, giving rise to true dicentric chromosomes. The centromere inactivation is clonally inherited, providing a new example of epigenetic chromosome imprinting and the possibility of genetically dissecting this process. The involvement of telomere-related sequences in centromere function is discussed. |
| Databáze: | OpenAIRE |
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