Aging-dependent expression of synapse-related proteins in the mouse brain
| Autor: | Shotaro Sakakibara, Kenji Mandai, Yoshimi Takai, Tomohiko Maruo, Muneaki Miyata, Hideki Mochizuki, Hajime Shiotani |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging Chemical synapse Nectins Biology Synaptic vesicle Presynapse Postsynapse Synapse 03 medical and health sciences Mice 0302 clinical medicine Postsynaptic potential Genetics medicine Animals Cell adhesion molecule Brain Gene Expression Regulation Developmental Membrane Proteins Cell Biology Cadherins Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Synapses Neuron Cell Adhesion Molecules Disks Large Homolog 4 Protein Guanylate Kinases 030217 neurology & neurosurgery |
| Zdroj: | Genes to cells : devoted to molecularcellular mechanisms. 22(5) |
| ISSN: | 1365-2443 |
| Popis: | A synapse is a cell adhesion structure that permits a neuron to pass a chemical or electrical signal to another neuron. They connect neurons and form neural networks that are essential for brain functions, such as learning and memory. At a chemical synapse, the presynapse and the postsynapse are connected by cell adhesion molecules. The presynapse contains synaptic vesicles and their release machinery, whereas the postsynapse contains postsynaptic densities and receptors for the neurotransmitters. Many proteins constituting a synapse have been identified, but their life-span expression profiles remain elusive. Here, we investigated the expression levels of representative synapse-related proteins by Western blot using the extranuclear supernatant fraction of the brains of mice at various ages. These proteins were classified into seven groups depending on their expression profiles during the embryonic stage, those from postnatal day 6 (P6) to P30, and those after P90. The expression levels of the majority of the proteins were gradually increased from the embryonic stage and then decreased at P14 or P30. After P90, the expression levels were not markedly changed or, in some proteins, increased. These results indicate that the expression levels of the synapse-related proteins are regulated orderly in an aging-dependent manner. |
| Databáze: | OpenAIRE |
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