Atrophy of primary lymphoid organs induced by Marek’s Disease Virus during early infection is associated with increased apoptosis, inhibition of cell proliferation and a severe B- lymphopenia

Autor: Caroline Denesvre, Camille Berthault, Thibaut Larcher, Sonja Härtle, Jean-François Vautherot, Laëtitia Trapp-Fragnet
Přispěvatelé: Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Department of Veterinary Science, Ludwig Maximilians University of Munich, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), ERA-NET Anihwa program, MADISUP project consortium, INRA and Region Centre-Val de Loire, ProdInra, Migration, Denesvre, Caroline
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
animal structures
Lymphoid Tissue
medicine.medical_treatment
Virologie
viruses
animal diseases
poulet
Apoptosis
Virus
03 medical and health sciences
Atrophy
thymus
Virology
hemic and lymphatic diseases
Lymphopenia
medicine
Marek Disease
Animals
Bursa of Fabricius
Herpesvirus 2
Gallid
Poultry Diseases
ComputingMilieux_MISCELLANEOUS
Cell Proliferation
prolifération cellulaire
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Marek's disease
lcsh:Veterinary medicine
atrophie
General Veterinary
biology
virus diseases
Immunosuppression
biology.organism_classification
medicine.disease
3. Good health
030104 developmental biology
Lymphatic system
Lytic cycle
bourse de fabricius
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
virus maladie de marek
lcsh:SF600-1100
Chickens
Research Article
Zdroj: 11. Symposium of the French Domestic Animal Immunology Network (IAD 2018)
11. Symposium of the French Domestic Animal Immunology Network (IAD 2018), Mar 2018, Tours, France. 56 p
Veterinary Research
Veterinary Research, BioMed Central, 2018, 49 (1), pp.1-18. ⟨10.1186/s13567-018-0526-x⟩
Veterinary Research 1 (49), 1-18. (2018)
Veterinary Research, Vol 49, Iss 1, Pp 1-18 (2018)
ISSN: 0928-4249
1297-9716
DOI: 10.1186/s13567-018-0526-x⟩
Popis: Marek’s disease is a multi-faceted highly contagious disease affecting chickens caused by the Marek’s disease alphaherpesvirus (MDV). MDV early infection induces a transient immunosuppression, which is associated with thymus and bursa of Fabricius atrophy. Little is known about the cellular processes involved in primary lymphoid organ atrophy. Here, by in situ TUNEL assay, we demonstrate that MDV infection results in a high level of apoptosis in the thymus and bursa of Fabricius, which is concomitant to the MDV lytic cycle. Interestingly, we observed that in the thymus most of the MDV infected cells at 6 days post-infection (dpi) were apoptotic, whereas in the bursa of Fabricius most of the apoptotic cells were uninfected suggesting that MDV triggers apoptosis by two different modes in these two primary lymphoid organs. In addition, a high decrease of cell proliferation was observed from 6 to 14 dpi in the bursa of Fabricius follicles, and not in the thymus. Finally, with an adapted absolute blood lymphocyte count, we demonstrate a major B-lymphopenia during the two 1st weeks of infection, and propose this method as a potent non-invasive tool to diagnose MDV bursa of Fabricius infection and atrophy. Our results demonstrate that the thymus and bursa of Fabricius atrophies are related to different cell mechanisms, with different temporalities, that affect infected and uninfected cells. Electronic supplementary material The online version of this article (10.1186/s13567-018-0526-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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