Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily
| Autor: | M. Cartiaux, Ismaïl Iraqui, Stephan Vissers, Antonio Urrestarazu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Transamination Autonomously replicating sequence Phenylalanine Recombinant Fusion Proteins Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Lyases Sequence Homology Substrate Specificity Fungal Proteins chemistry.chemical_compound Ammonia Gene Expression Regulation Fungal Consensus Sequence Genetics Aromatic amino acids Urea Amino Acid Sequence Amino Acids Cloning Molecular DNA Fungal Molecular Biology Peptide sequence Transaminases Amino acid synthesis chemistry.chemical_classification Base Sequence biology Genetic Complementation Test Structural gene biology.organism_classification Culture Media Amino acid Isoenzymes chemistry Biochemistry Enzyme Induction Multigene Family Tyrosine 2-Aminoadipic Acid Sequence Alignment Gene Deletion |
| Zdroj: | Molecular and General Genetics MGG. 257:238-248 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/s004380050644 |
| Popis: | The ARO8 and ARO9 genes of Saccharomyces cerevisiae were isolated by complementation of the phenylalanine/tyrosine auxotrophy of an aro8 and aro9 double-mutant strain that is defective in aromatic aminotransferase I (aro8) and II (aro9). The genes were sequenced, and deletion mutants were constructed and analysed. The expression of ARO8 and ARO9 was studied. The deduced amino acid sequences of Aro8p and Aro9p suggest that the former is a 500-residue, 56168-Da polypeptide and the latter a 513-residue, 58516-Da polypeptide. They correspond, respectively, to Ygl202p and Yhr137p, two putative proteins of unknown function revealed by systematic sequencing of the yeast genome. We show that aromatic aminotransferases I and II are homologous proteins, members of aminotransferase subgroup I, and, together with three other proteins, they constitute within the subgroup a new subfamily of enzymes specialised for aromatic amino acid and alpha-aminoadipate transamination. ARO8 expression is subject to the general control of amino acid biosynthesis. ARO9 expression is induced when aromatic amino acids are present in the growth medium and also in aro8 mutants grown on minimal ammonia medium. An autonomously replicating sequence (ARS) element is located between the ARO8 gene and YGL201c which encodes a protein of the minichromosome maintenance family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink