| Autor: |
Sae-Lee, Chanachai, Barrow, Timothy M., Colicino, Elena, Choi, Si Ho, Rabanal-Ruiz, Yoana, Green, Daniel, Korolchuk, Viktor I., Mathers, John C., Byun, Hyang-Min |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.20522577 |
| Popis: |
Additional file 1. Supplementary Table 1. Amino acid sequences of DNMT isoforms; Supplementary Table 2. Frequency of gains and losses of methylation by DNMT isoform overexpression; Supplementary Table 3. KEGG pathway analysis for loci hypermethylated in response to DNMT isoform overexpression; Supplementary Table 4. Primers used for analysis of exogenous DNMT isoform expression; Supplementary Figure 1. Endogenous expression of DNMT isoforms in HEK293T cells. Expression was assessed in control cells by qPCR. Due to high sequence similarity between some isoforms (DNMT3B1-5, DNMTD3B1/2, and DNMTD3B3/4), expression levels for individual isoforms could not be determined; Supplementary Figure 2. Gains of methylation in relation to CpG density. Frequency of gains of methylation (Db>0.2) at CpG sites mapping to CpG islands (green), shores (yellow), shelves (brown) and open seas (blue) by DNMT isoform; Supplementary Figure 3. Verification of target loci by pyrosequencing. Six loci identified as targets of DNMT1 (cg09559735), DNMT3A2 (cg02732111), DNMTD3B2 (cg25533247), DNMTD3B3 (cg08927738, cg12150401), DNMTD3B4 (cg22976313) and DNMT3L (cg08927738, cg12150401) were interrogated in DNA extracted from overexpressing and control (‘Myc’) cells. Beta values from the Illumina Infinium EPIC microarray were converted to percentages for comparison; Supplementary Figure 4. Density plot of genome-wide CpG methylation (black) and on chrX (pink). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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