SynGAP Regulates Spine Formation
| Autor: | Luis Vazquez, Irene Knuesel, Irina Sokolova, Hong Jung Chen, Mary B. Kennedy |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Dendritic Spines
Mutant Synaptogenesis Nerve Tissue Proteins AMPA receptor Hippocampal formation SYNGAP1 Biology Hippocampus Mice Animals Cells Cultured Mice Knockout Neurons General Neuroscience Intracellular Signaling Peptides and Proteins Glutamate receptor Wild type Excitatory Postsynaptic Potentials Membrane Proteins Synapsin Protein Structure Tertiary Cell biology Phenotype nervous system ras GTPase-Activating Proteins Disks Large Homolog 4 Protein Guanylate Kinases Cellular/Molecular |
| Zdroj: | The Journal of Neuroscience. 24:8862-8872 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.3213-04.2004 |
| Popis: | SynGAP is a brain-specific ras GTPase-activating protein that is an abundant component of the signaling complex associated with the NMDA-type glutamate receptor. We generated mutant mice lacking synGAP to study its physiological role. Homozygous mutant mice die in the first few days after birth; however, neurons from mutant embryos can be maintained in culture. Here, we report that spine and synapse formation are accelerated in cultured mutant neurons, and the spines of mature mutant neurons are significantly larger than those of wild type. Clusters of PSD-95 and subunits of AMPA-type and NMDA-type glutamate receptors accumulate in spines of mutant neurons by day 10in vitro, whereas in wild-type neurons they are still mostly located in dendritic shafts. The frequency and amplitude of miniature EPSCs are larger in mutant neurons at day 10in vitro, confirming that they have more functional synapses. At day 21in vitro, the spines of mutant neurons remain significantly larger than those of wild type. The mutant phenotype at day 10in vitrocan be rescued by introduction of recombinant wild-type synGAP on day 9. In contrast, introduction of mutant synGAP with a mutated GAP domain or lacking the terminal domain that binds to PSD-95 does not rescue the mutant phenotype, indicating that both domains play a role in control of spine formation. Thus, the GAP activity of synGAP and its association with PSD-95 are important for normal regulation of spine and synapse formation in hippocampal neurons. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|