Human Kupffer cells are cytotoxic against human colon adenocarcinoma
| Autor: | S. A. Curley, Caroline O. Oyedeji, Jim Klostergaard, Raphael E. Pollock, M.E. Leroux, Li Wang, Mark S. Roh |
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| Rok vydání: | 1990 |
| Předmět: |
Cytotoxicity
Immunologic Lipopolysaccharide Cell Survival Kupffer Cells Adenocarcinoma chemistry.chemical_compound Tumor Cells Cultured Humans Medicine Cytotoxic T cell Secretion Cytotoxicity Tumor Necrosis Factor-alpha business.industry Kupffer cell medicine.disease Monokine medicine.anatomical_structure chemistry Colonic Neoplasms Immunology Cancer research Surgery Tumor necrosis factor alpha business |
| Zdroj: | British Journal of Surgery. 77:1066-1070 |
| ISSN: | 1365-2168 0007-1323 |
| Popis: | Colorectal liver metastases are a common clinical problem and require more effective therapy. Kupffer cells (KC) perform many important homeostatic functions within the liver and may also possess the ability to mediate tumor cytotoxicity. We investigated the ability of human KC to mediate cytotoxicity against human colon adenocarcinoma targets (HT 29) in vitro. Unstimulated human KC were cytotoxic against the HT 29 targets at all effector/target ratios tested. This cytotoxicity was increased significantly (p < 0.05) when the KC were stimulated with interferon-γ and lipopolysaccharide. Human KC produced tumor necrosis factor (TNF), and KC stimulation significantly (p < 0.05) increased secretion of this monokine. The addition of anti-TNF antibody to the KC-HT 29 cocultures completely neutralized all of the available TNF yet cytotoxicity was not affected, suggesting the participation of a membrane-bound form of TNF or other mechanisms. |
| Databáze: | OpenAIRE |
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