SCF increases cardiac stem cell migration through PI3K/AKT and MMP-2/-9 signaling
| Autor: | Wei Jie, Tian-Fa Li, Junli Guo, You-Ling Lan, Shaoli Guo, Mengsen Li, Yue-Qiong Kong, Zhihua Shen, Shaojiang Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
cell migration Morpholines Stem cell factor Biology matrix metalloproteinase-2 Rats Sprague-Dawley Phosphatidylinositol 3-Kinases Western blot Cell Movement matrix metalloproteinase-9 Genetics medicine Animals Enzyme Inhibitors Protein kinase B PI3K/AKT/mTOR pathway Cells Cultured Phosphoinositide-3 Kinase Inhibitors stem cell factor (SCF)/c-Kit system Stem Cell Factor medicine.diagnostic_test Myocardium Stem Cells cardiac stem cells Cell migration General Medicine Articles Rats Transplantation Proto-Oncogene Proteins c-kit Gene Expression Regulation Matrix Metalloproteinase 9 Chromones Cancer research Matrix Metalloproteinase 2 Stem cell Signal transduction PI3K/AKT signaling Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | The transplantation of cardiac stem cells (CSCs) is thought to be responsible for improving the performance of injured heart induced by myocardial infarction (MI). However, the mechanisms involved in the migration of activated CSCs post-MI remain to be clarified. In this study, CSCs were isolated from rat hearts and a cellular migration assay was performed using a 24-well Transwell system. Stem cell factor (SCF) induced CSC migration in a concentration-dependent manner, which could be blocked with an SCF antibody as well as a PI3K/AKT inhibitor, LY294002. Moreover, SCF induced the expression and activity of matrix metalloproteinase (MMP)-2 and MMP-9 in a concentration- and time-dependent manner, as measured by quantitative RT-PCR, western blot analysis and gelatin zymography. Results of western blot analysis revealed phosphorylated AKT was markedly increased in SCF-treated CSCs and that inhibition of SCF/c-Kit signaling or phospho-AKT activity significantly attenuated the SCF-induced expression of MMP-2 and MMP-9. Thus, our results showed that SCF partially mediated CSC migration via the activation of PI3K/AKT/MMP-2/-9 signaling. |
| Databáze: | OpenAIRE |
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