Design Considerations for RNA Spherical Nucleic Acids (SNAs)
| Autor: | Kevin L. Kohlstedt, Alyssa B. Chinen, Stacey N. Barnaby, Chad A. Mirkin, George C. Schatz, Grant A. Perelman |
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| Rok vydání: | 2016 |
| Předmět: |
Models
Molecular RNA Stability Cell division Surface Properties Biomedical Engineering Metal Nanoparticles Pharmaceutical Science Bioengineering Sequence (biology) 02 engineering and technology 010402 general chemistry 01 natural sciences Article Moiety Pharmacology Modularity (networks) Base Sequence Oligonucleotide Chemistry Organic Chemistry RNA 021001 nanoscience & nanotechnology 0104 chemical sciences Biochemistry Drug Design Nucleic acid Nucleic Acid Conformation Gold 0210 nano-technology Biotechnology |
| Zdroj: | Bioconjugate Chemistry |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/acs.bioconjchem.6b00350 |
| Popis: | Ribonucleic acids (RNAs) are key components in many cellular processes such as cell division, differentiation, growth, aging, and death. RNA spherical nucleic acids (RNA-SNAs), which consist of dense shells of double-stranded RNA on nanoparticle surfaces, are powerful and promising therapeutic modalities because they confer advantages over linear RNA such as high cellular uptake and enhanced stability. Due to their three-dimensional shell of oligonucleotides, SNAs, in comparison to linear nucleic acids, interact with the biological environment in unique ways. Herein, the modularity of the RNA-SNA is used to systematically study structure–function relationships in order to understand how the oligonucleotide shell affects interactions with a specific type of biological environment, namely, one that contains serum nucleases. We use a combination of experiment and theory to determine the key architectural properties (i.e., sequence, density, spacer moiety, and backfill molecule) that affect how RNA-SNAs interact with serum nucleases. These data establish a set of design parameters for SNA architectures that are optimized in terms of stability. |
| Databáze: | OpenAIRE |
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